Ser427
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Home > Phosphorylation Site Page: > Ser427  -  p70S6K (mouse)

Site Information
ESVKEKFsFEPKIRs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 450014

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 ) , mass spectrometry ( 1 , 2 , 4 , 5 ) , peptide sequencing ( 5 ) , phosphoamino acid analysis ( 5 ) , phosphopeptide mapping ( 5 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 1 , 2 , 4 ) , Swiss 3T3 (fibroblast) ( 5 )

Upstream Regulation
Treatments:
GSK2334470 ( 2 ) , insulin ( 2 , 4 ) , LY294002 ( 2 , 4 ) , MK-2206 ( 2 , 4 ) , Torin1 ( 2 )

References 

1

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

2

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

3

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

4

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

5

Pearson RB, et al. (1995) The principal target of rapamycin-induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain. EMBO J 14, 5279-87
7489717   Curated Info