Thr252
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Home > Phosphorylation Site Page: > Thr252  -  p70S6K (mouse)

Site Information
HDGtVtHtFCGtIEY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448606

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , mass spectrometry ( 3 , 4 , 8 ) , peptide sequencing ( 8 ) , phospho-antibody ( 1 , 2 , 5 , 6 , 7 ) , phosphoamino acid analysis ( 8 ) , phosphopeptide mapping ( 8 ) , western blotting ( 1 , 2 , 5 , 6 , 7 )
Disease tissue studied:
breast cancer ( 2 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 5 ) , 'stem, embryonic' ( 5 ) , adipose tissue ( 5 ) , brain ( 3 , 4 ) , breast [PDK1 (mouse), homozygous knockout] ( 2 ) , CHO (fibroblast) [Tau iso8 (human)] ( 7 ) , embryo ( 3 ) , heart ( 5 ) , liver ( 3 , 5 ) , MEF (fibroblast) ( 6 ) , sertoli-testis ( 1 ) , Swiss 3T3 (fibroblast) ( 8 )

Upstream Regulation
Regulatory protein:
mTOR (mouse) ( 1 ) , PDK1 (human) ( 2 ) , PDK1 (mouse) ( 5 ) , RICTOR (mouse) ( 6 )
Treatments:
insulin ( 5 , 6 ) , meal feeding ( 5 ) , rapamycin ( 7 ) , troglitazone ( 7 ) , wortmannin ( 7 )

References 

1

Boyer A, et al. (2016) mTOR Regulates Gap Junction Alpha-1 Protein Trafficking in Sertoli Cells and Is Required for the Maintenance of Spermatogenesis in Mice. Biol Reprod 95, 13
27281705   Curated Info

2

Du J, et al. (2016) PDK1 promotes tumor growth and metastasis in a spontaneous breast cancer model. Oncogene 35, 3314-23
26455327   Curated Info

3

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

4

Guo A (2011) CST Curation Set: 12477; Year: 2011; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

5

Bayascas JR, et al. (2008) Mutation of the PDK1 PH domain inhibits protein kinase B/Akt, leading to small size and insulin resistance. Mol Cell Biol 28, 3258-72
18347057   Curated Info

6

Guertin DA, et al. (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11, 859-71
17141160   Curated Info

7

d'Abramo C, Ricciarelli R, Pronzato MA, Davies P (2006) Troglitazone, a peroxisome proliferator-activated receptor-gamma agonist, decreases tau phosphorylation in CHOtau4R cells. J Neurochem 98, 1068-77
16787414   Curated Info

8

Pearson RB, et al. (1995) The principal target of rapamycin-induced p70s6k inactivation is a novel phosphorylation site within a conserved hydrophobic domain. EMBO J 14, 5279-87
7489717   Curated Info