Ser228
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Home > Phosphorylation Site Page: > Ser228  -  PINK1 (human)

Site Information
MWNISAGsSSEAILN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 25792200

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 2 ) , immunoprecipitation ( 2 , 7 ) , mass spectrometry ( 2 , 7 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ) , phospho-antibody ( 2 ) , western blotting ( 2 , 3 , 4 , 6 , 7 )
Disease tissue studied:
Parkinson's disease ( 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 7 ) , COS (fibroblast) ( 4 ) , heart ( 2 ) , HEK293T (epithelial) ( 4 ) , HeLa (cervical) ( 1 , 3 , 4 , 5 , 7 ) , MEF (fibroblast) ( 4 , 6 ) , myocyte-heart ( 2 )

Upstream Regulation
Putative in vivo kinases:
AMPKA2 (human) ( 2 ) , PINK1 (human) ( 1 , 2 , 6 , 7 )
Kinases, in vitro:
PINK1 (human) ( 4 )
Treatments:
CCCP ( 4 , 7 ) , lactacystin ( 4 )

Downstream Regulation
Effects of modification on PINK1:
enzymatic activity, induced ( 4 ) , intracellular localization ( 6 , 7 ) , phosphorylation ( 4 ) , ubiquitination ( 6 )

References 

1

Waters CS, Angenent SB, Altschuler SJ, Wu LF (2023) A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Cell Rep 42, 113260
37851575   Curated Info

2

Wang B, et al. (2017) AMPKα2 Protects Against the Development of Heart Failure by Enhancing Mitophagy via PINK1 Phosphorylation. Circ Res
29284690   Curated Info

3

Akabane S, et al. (2016) Constitutive Activation of PINK1 Protein Leads to Proteasome-mediated and Non-apoptotic Cell Death Independently of Mitochondrial Autophagy. J Biol Chem 291, 16162-74
27302064   Curated Info

4

Aerts L, Craessaerts K, De Strooper B, Morais VA (2015) PINK1 Kinase Catalytic Activity Is Regulated by Phosphorylation on Serines 228 and 402. J Biol Chem 290, 2798-811
25527497   Curated Info

5

Okatsu K, et al. (2013) A Dimeric PINK1-containing Complex on Depolarized Mitochondria Stimulates Parkin Recruitment. J Biol Chem 288, 36372-84
24189060   Curated Info

6

Iguchi M, et al. (2013) Parkin-catalyzed Ubiquitin-Ester Transfer Is Triggered by PINK1-dependent Phosphorylation. J Biol Chem 288, 22019-32
23754282   Curated Info

7

Okatsu K, et al. (2012) PINK1 autophosphorylation upon membrane potential dissipation is essential for Parkin recruitment to damaged mitochondria. Nat Commun 3, 1016
22910362   Curated Info

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