Ser117
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Home > Phosphorylation Site Page: > Ser117  -  PGM1 (human)

Site Information
GGIILtAsHNPGGPN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2525691

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 17 , 18 , 19 , 20 , 21 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 )
Disease tissue studied:
breast cancer ( 11 , 19 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , cervical cancer ( 35 ) , cervical adenocarcinoma ( 35 ) , leukemia ( 20 , 23 ) , acute myelogenous leukemia ( 20 , 23 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 18 ) , acute megakaryoblastic leukemia (M7) ( 18 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 18 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 18 ) , acute myeloblastic leukemia, without maturation (M1) ( 18 ) , hepatocellular carcinoma, surrounding tissue ( 33 ) , lung cancer ( 7 , 19 , 31 ) , non-small cell lung cancer ( 19 ) , non-small cell lung adenocarcinoma ( 7 ) , lymphoma ( 12 ) , B cell lymphoma ( 18 ) , Burkitt's lymphoma ( 12 ) , non-Hodgkin's lymphoma ( 18 ) , follicular lymphoma ( 12 ) , mantle cell lymphoma ( 12 ) , ovarian cancer ( 10 ) , pancreatic ductal adenocarcinoma ( 14 ) , multiple myeloma ( 18 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 29 , 37 ) , 'pancreatic, ductal'-pancreas ( 14 ) , 293E (epithelial) ( 27 ) , 293T (epithelial) ( 5 ) , 786-O (renal) ( 4 ) , 786-O (renal) [VHL (human), transfection] ( 4 ) , AML-193 (monocyte) ( 18 ) , BJAB (B lymphocyte) ( 12 ) , breast ( 2 ) , BT-20 (breast cell) ( 19 ) , BT-549 (breast cell) ( 19 ) , Calu 6 (pulmonary) ( 19 ) , CL1-0 (pulmonary) ( 31 ) , CL1-1 (pulmonary) ( 31 ) , CL1-2 (pulmonary) ( 31 ) , CL1-5 (pulmonary) ( 31 ) , CMK (megakaryoblast) ( 18 ) , CTS (myeloid) ( 18 ) , DG75 (B lymphocyte) ( 32 ) , DOHH2 ('B lymphocyte, precursor') ( 18 ) , FL-18 (B lymphocyte) ( 12 ) , FL-318 (B lymphocyte) ( 12 ) , Flp-In T-Rex-293 (epithelial) ( 21 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 21 ) , H2009 (pulmonary) ( 19 ) , H2077 (pulmonary) ( 19 ) , H2887 (pulmonary) ( 19 ) , H322 (pulmonary) ( 19 ) , H322M (pulmonary) ( 19 ) , HCC1359 (pulmonary) ( 19 ) , HCC1937 (breast cell) ( 19 ) , HCC2279 (pulmonary) ( 19 ) , HCC366 (pulmonary) ( 19 ) , HCC4006 (pulmonary) ( 19 ) , HCC78 (pulmonary) ( 19 ) , HCC827 (pulmonary) ( 19 ) , HEL (erythroid) ( 18 ) , HeLa (cervical) ( 1 , 9 , 17 , 24 , 36 , 38 , 40 , 41 , 43 , 44 ) , HeLa S3 (cervical) ( 35 ) , hepatocyte-liver ( 33 ) , HMLER ('stem, breast cancer') ( 11 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 11 ) , HOP62 (pulmonary) ( 19 ) , HUES-9 ('stem, embryonic') ( 30 ) , JEKO-1 (B lymphocyte) ( 12 ) , Jurkat (T lymphocyte) ( 15 , 25 , 26 , 28 , 39 , 42 ) , Kasumi-1 (myeloid) ( 18 ) , KG-1 (myeloid) ( 18 , 23 ) , LCLC-103H (pulmonary) ( 19 ) , leukocyte-blood ( 34 ) , LOU-NH91 (squamous) ( 19 ) , MCF-7 (breast cell) ( 19 ) , MDA-MB-231 (breast cell) ( 19 ) , MDA-MB-468 (breast cell) ( 19 ) , MV4-11 (macrophage) ( 18 , 20 ) , NCEB-1 (B lymphocyte) ( 12 ) , NCI-H1395 (pulmonary) ( 19 ) , NCI-H1568 (pulmonary) ( 19 ) , NCI-H157 (pulmonary) ( 19 ) , NCI-H1648 (pulmonary) ( 19 ) , NCI-H1666 (pulmonary) ( 19 ) , NCI-H2030 (pulmonary) ( 19 ) , NCI-H2172 (pulmonary) ( 19 ) , NCI-H460 (pulmonary) ( 19 ) , NCI-H520 (squamous) ( 19 ) , NCI-H647 (pulmonary) ( 19 ) , OCI-ly1 (B lymphocyte) ( 12 ) , OPM-2 (plasma cell) ( 18 ) , ovary ( 10 ) , P31/FUJ (erythroid) ( 18 , 20 ) , PC9 (pulmonary) ( 7 , 19 ) , Raji (B lymphocyte) ( 12 ) , RAMOS (B lymphocyte) ( 12 ) , REC-1 (B lymphocyte) ( 12 ) , RL ('B lymphocyte, precursor') ( 18 ) , RPMI-8226 (plasma cell) ( 18 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 13 ) , SU-DHL-4 (B lymphocyte) ( 12 ) , SU-DHL-6 (B lymphocyte) ( 18 ) , U266 (plasma cell) ( 18 ) , UPN-1 (B lymphocyte) ( 12 ) , WM239A (epidermal) ( 6 )

Upstream Regulation
Treatments:
BI_4834 ( 24 ) , EGF ( 36 ) , metastatic potential ( 31 ) , U0126 ( 36 )

References 

1

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
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3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
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4

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
26506913   Curated Info

5

Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23
25278378   Curated Info

6

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

7

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

8

Lee Y, Stiers KM, Kain BN, Beamer LJ (2014) Compromised catalysis and potential folding defects in in vitro studies of missense mutants associated with hereditary phosphoglucomutase 1 deficiency. J Biol Chem 289, 32010-9
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9

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

10

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

11

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

12

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
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13

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

14

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
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15

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
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16

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

17

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
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18

Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37
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19

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

20

Alcolea MP, et al. (2012) Phosphoproteomic analysis of leukemia cells under basal and drug-treated conditions identifies markers of kinase pathway activation and mechanisms of resistance. Mol Cell Proteomics 11, 453-66
22547687   Curated Info

21

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
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22

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

23

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

24

Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540
21857030   Curated Info

25

Mulhern D (2011) CST Curation Set: 12710; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

26

Mulhern D (2011) CST Curation Set: 12711; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

27

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

28

Guo A (2011) CST Curation Set: 11984; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]XP
Curated Info

29

Zhao X, et al. (2011) Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes. Mol Cell Proteomics 10, M110.000299
20833797   Curated Info

30

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

31

Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97
20815410   Curated Info

32

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

33

Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9
20166139   Curated Info

34

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
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35

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

36

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
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37

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
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38

Nagano K, et al. (2009) Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment. Proteomics 9, 2861-74
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39

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
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40

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
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41

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
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42

Stokes M (2008) CST Curation Set: 3885; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

43

Imami K, et al. (2008) Automated Phosphoproteome Analysis for Cultured Cancer Cells by Two-Dimensional NanoLC-MS Using a Calcined Titania/C18 Biphasic Column. Anal Sci 24, 161-6
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44

Yu LR, et al. (2007) Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra. J Proteome Res 6, 4150-62
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