Ser345
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Home > Phosphorylation Site Page: > Ser345  -  MLKL (mouse)

Site Information
ELSKTQNsIsRtAKS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 22996309

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 12 ) , immunoassay ( 4 ) , immunoprecipitation ( 4 , 6 , 9 ) , mass spectrometry ( 4 ) , mass spectrometry (in vitro) ( 12 ) , mutation of modification site ( 2 , 9 , 10 , 12 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 11 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 )
Disease tissue studied:
atherosclerosis ( 11 ) , colorectal cancer ( 2 , 8 , 9 , 10 ) , colorectal carcinoma ( 2 , 9 , 10 ) , lymphoma ( 2 , 10 ) , fibrosarcoma of soft tissue ( 2 , 9 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) ( 2 , 9 ) , aorta ( 11 ) , CT26 (epithelial) ( 8 ) , fibroblast-embryo ( 9 ) , fibroblast-skin ( 2 , 10 , 12 ) , HeLa (cervical) ( 9 ) , HT-29 (intestinal) ( 2 , 9 , 10 ) , L929 (fibroblast) ( 2 , 9 ) , macrophage-bone marrow ( 3 , 4 , 5 ) , MEF (fibroblast) ( 4 , 6 , 7 ) , SVEC (endothelial) ( 9 ) , U-937 (myeloid) ( 2 , 10 )

Upstream Regulation
Regulatory protein:
FADD (mouse) ( 8 ) , FAM105B (human) ( 4 ) , IFNB1 (mouse) ( 5 ) , RIPK3 (mouse) ( 8 ) , SPATA2 (mouse) ( 7 ) , STING (mouse) ( 5 )
Putative in vivo kinases:
RIPK3 (human) ( 9 )
Kinases, in vitro:
RIPK3 (mouse) ( 12 )
Treatments:
GSK'872 ( 9 ) , GW440139B ( 9 ) , LPS ( 5 ) , SM-164 ( 6 ) , TNF ( 4 , 6 , 9 ) , Z-VAD-FMK ( 3 , 4 , 5 , 6 , 9 )

Downstream Regulation
Effects of modification on MLKL:
activity, induced ( 9 ) , intracellular localization ( 9 ) , protein conformation ( 9 ) , protein stabilization ( 4 )
Effects of modification on biological processes:
apoptosis, induced ( 2 , 4 )

References 

1

Fritsch M, et al. (2019) Caspase-8 is the molecular switch for apoptosis, necroptosis and pyroptosis. Nature 575, 683-687
31748744   Curated Info

2

Moerke C, et al. (2018) The anticonvulsive Phenhydan suppresses extrinsic cell death. Cell Death Differ
30442947   Curated Info

3

Sarhan J, et al. (2018) Caspase-8 induces cleavage of gasdermin D to elicit pyroptosis during infection. Proc Natl Acad Sci U S A 115, E10888-E10897
30381458   Curated Info

4

Heger K, et al. (2018) OTULIN limits cell death and inflammation by deubiquitinating LUBAC. Nature 559, 120-124
29950720   Curated Info

5

Sarhan J, et al. (2018) Constitutive interferon signaling maintains critical threshold of MLKL expression to license necroptosis. Cell Death Differ
29786074   Curated Info

6

Wang H, et al. (2017) PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A 114, 11944-11949
29078411   Curated Info

7

Wei R, et al. (2017) SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination. Genes Dev 31, 1162-1176
28701375   Curated Info

8

Aaes TL, et al. (2016) Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity. Cell Rep 15, 274-87
27050509   Curated Info

9

Rodriguez DA, et al. (2016) Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis. Cell Death Differ 23, 76-88
26024392   Curated Info

10

Tanzer MC, et al. (2015) Necroptosis signalling is tuned by phosphorylation of MLKL residues outside the pseudokinase domain activation loop. Biochem J 471, 255-65
26283547   Curated Info

11

Meng L, Jin W, Wang X (2015) RIP3-mediated necrotic cell death accelerates systematic inflammation and mortality. Proc Natl Acad Sci U S A 112, 11007-12
26283397   Curated Info

12

Murphy JM, et al. (2013) The pseudokinase MLKL mediates necroptosis via a molecular switch mechanism. Immunity 39, 443-53
24012422   Curated Info