Lys420

Site Information
GsVtkkRkLEstEsR SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 25031403

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 2 ) , mass spectrometry ( 1 , 3 ) , mutation of modification site ( 2 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 3 ) , COS7 (fibroblast) ( 2 ) , HeLa (cervical) [SUMO2 (human), transfection, SUMO2 K0 Q87R (lysine-deficient SUMO2, with Q87R to create convenient Trypsin cleavage site)] ( 1 ) , HeLa (cervical) ( 2 )

Upstream Regulation
Treatments:	As2O3 ( 3 )

References
1	Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36 25218447 Curated Info
2	Simon DN, Domaradzki T, Hofmann WA, Wilson KL (2013) Lamin A tail modification by SUMO1 is disrupted by familial partial lipodystrophy-causing mutations. Mol Biol Cell 24, 342-50 23243001 Curated Info
3	Galisson F, et al. (2011) A novel proteomics approach to identify SUMOylated proteins and their modification sites in human cells. Mol Cell Proteomics 10, M110.004796 21098080 Curated Info