Ser435
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Home > Phosphorylation Site Page: > Ser435  -  RIC8A (rat)

Site Information
GRPEGQYsEDEDtDT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 468627

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 3 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 3 , 4 , 5 , 6 ) , mass spectrometry (in vitro) ( 1 ) , phospho-antibody ( 3 ) , western blotting ( 3 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 4 ) , 'brain, cerebellum' ( 4 ) , 'brain, cerebral cortex' ( 4 ) , 'kidney, cortex' ( 5 ) , adipose tissue ( 4 ) , blood ( 4 ) , heart ( 4 ) , HEK293T (epithelial) ( 3 ) , intestine ( 4 ) , kidney ( 4 ) , liver ( 4 , 6 ) , lung ( 4 ) , muscle ( 4 ) , pancreas ( 4 ) , spleen ( 4 ) , stomach ( 4 ) , testis ( 4 ) , thymus ( 4 )

Upstream Regulation
Kinases, in vitro:
CK2A1 (human) ( 3 ) , CK2A1 (rat) ( 2 )

Downstream Regulation
Effects of modification on RIC8A:
activity, induced ( 2 ) , molecular association, regulation ( 1 , 3 ) , protein conformation ( 2 ) , protein stabilization ( 1 )
Induce interaction with:
G-alpha i1 (rat) ( 3 )

References 

1

Srivastava D, Gakhar L, Artemyev NO (2019) Structural underpinnings of Ric8A function as a G-protein α-subunit chaperone and guanine-nucleotide exchange factor. Nat Commun 10, 3084
31300652   Curated Info

2

Zeng B, et al. (2019) Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A. Structure 27, 1137-1147.e5
31155309   Curated Info

3

Papasergi-Scott MM, et al. (2018) Dual phosphorylation of Ric-8A enhances its ability to mediate G protein α subunit folding and to stimulate guanine nucleotide exchange. Sci Signal 11
29844055   Curated Info

4

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

5

Feric M, et al. (2011) Large-scale phosphoproteomic analysis of membrane proteins in renal proximal and distal tubule. Am J Physiol Cell Physiol 300, C755-70
21209370   Curated Info

6

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

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