Thr243
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Home > Phosphorylation Site Page: > Thr243  -  STLK3 (mouse)

Site Information
tRNKVRKtFVGtPCW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456505

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 5 , 6 , 8 ) , mutation of modification site ( 2 , 7 , 9 , 10 ) , phospho-antibody ( 3 , 4 ) , western blotting ( 3 , 4 )
Relevant cell line - cell type - tissue:
brain ( 5 ) , embryo ( 5 ) , kidney ( 2 , 3 , 4 ) , kidney [genetic knockin] ( 7 ) , liver ( 5 , 6 ) , lung ( 8 ) , oocyte ( 9 ) , oocyte [CPEB (mouse)] ( 10 )

Upstream Regulation
Regulatory protein:
UMOD (mouse) ( 3 ) , WNK4 (mouse) ( 4 )
Putative in vivo kinases:
WNK4 (mouse) ( 9 )
Kinases, in vitro:
STLK3 (mouse) ( 10 )

Downstream Regulation
Effects of modification on STLK3:
enzymatic activity, induced ( 7 , 9 , 10 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

CastaƱeda-Bueno M, et al. (2014) Modulation of NCC activity by low and high K+ intake: insights into the signaling pathways involved. Am J Physiol Renal Physiol 306, F1507-19
24761002   Curated Info

3

Trudu M, et al. (2013) Common noncoding UMOD gene variants induce salt-sensitive hypertension and kidney damage by increasing uromodulin expression. Nat Med 19, 1655-60
24185693   Curated Info

4

CastaƱeda-Bueno M, et al. (2012) Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process. Proc Natl Acad Sci U S A 109, 7929-34
22550170   Curated Info

5

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

6

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

7

Chiga M, et al. (2011) Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade. J Cell Sci 124, 1391-5
21486947   Curated Info

8

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

9

Gagnon KB, Delpire E (2010) On the substrate recognition and negative regulation of SPAK, a kinase modulating Na+-K+-2Cl- cotransport activity. Am J Physiol Cell Physiol 299, C614-20
20463172   Curated Info

10

Gagnon KB, England R, Delpire E (2006) Characterization of SPAK and OSR1, regulatory kinases of the Na-K-2Cl cotransporter. Mol Cell Biol 26, 689-98
16382158   Curated Info