Thr185
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Home > Phosphorylation Site Page: > Thr185  -  OSR1 (mouse)

Site Information
TRNKVRKtFVGtPCW   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456503

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry ( 6 , 7 ) , mutation of modification site ( 2 , 8 ) , phospho-antibody ( 2 , 5 ) , western blotting ( 2 , 5 )
Relevant cell line - cell type - tissue:
brain ( 6 ) , embryo ( 6 ) , kidney ( 5 ) , kidney [genetic knockin] ( 8 ) , liver ( 6 , 7 ) , T lymphocyte ( 2 )

Upstream Regulation
Regulatory protein:
UMOD (mouse) ( 5 )
Putative in vivo kinases:
WNK1 (mouse) ( 2 )
Treatments:
hypotonic_buffer ( 9 )

Downstream Regulation
Effects of modification on OSR1:
enzymatic activity, induced ( 8 )
Effects of modification on biological processes:
cell motility, induced ( 2 )

References 

1

Zheng S, et al. (2018) Regulation of erythrocyte Na/K/2Cl cotransport by an oxygen-switched kinase cascade. J Biol Chem
30563844   Curated Info

2

Köchl R, et al. (2016) WNK1 kinase balances T cell adhesion versus migration in vivo. Nat Immunol 17, 1075-83
27400149   Curated Info

3

Begum G, et al. (2015) Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke. Stroke 46, 1956-65
26069258   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Trudu M, et al. (2013) Common noncoding UMOD gene variants induce salt-sensitive hypertension and kidney damage by increasing uromodulin expression. Nat Med 19, 1655-60
24185693   Curated Info

6

Stokes MP, et al. (2012) PTMScan Direct: Identification and Quantification of Peptides from Critical Signaling Proteins by Immunoaffinity Enrichment Coupled with LC-MS/MS. Mol Cell Proteomics 11, 187-201
22322096   Curated Info

7

Guo A (2011) CST Curation Set: 12478; Year: 2011; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]
Curated Info

8

Chiga M, et al. (2011) Phenotypes of pseudohypoaldosteronism type II caused by the WNK4 D561A missense mutation are dependent on the WNK-OSR1/SPAK kinase cascade. J Cell Sci 124, 1391-5
21486947   Curated Info

9

Richardson C, et al. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci 121, 675-84
18270262   Curated Info