Ser289
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Home > Phosphorylation Site Page: > Ser289  -  MDM4 (human)

Site Information
DDLEDSKsLSDDTDV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 456471

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 1 ) , [32P] bio-synthetic labeling ( 4 ) , immunoprecipitation ( 2 , 3 ) , mutation of modification site ( 3 , 4 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
bone cancer ( 2 ) , breast cancer ( 2 ) , lung cancer ( 1 , 2 , 3 ) , non-small cell lung cancer ( 1 , 2 , 3 ) , non-small cell lung adenocarcinoma ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CK1A (human) ( 3 , 4 )
Kinases, in vitro:
CK1A (human) ( 1 , 4 )
Treatments:
siRNA ( 3 )

Downstream Regulation
Effects of modification on MDM4:
molecular association, regulation ( 2 , 3 , 4 )
Effects of modification on biological processes:
transcription, inhibited ( 3 )
Induce interaction with:
MDM2 (human) ( 3 ) , p53 (human) ( 3 )

References 

1

Liu X, et al. (2019) Tumor-derived CK1α mutations enhance MDMX inhibition of p53. Oncogene
31462704   Curated Info

2

Wei X, et al. (2016) Secondary interaction between MDMX and p53 core domain inhibits p53 DNA binding. Proc Natl Acad Sci U S A 113, E2558-63
27114532   Curated Info

3

Wu S, et al. (2012) Casein Kinase 1α Regulates an MDMX Intramolecular Interaction To Stimulate p53 Binding. Mol Cell Biol 32, 4821-32
23028042   Curated Info

4

Chen L, Li C, Pan Y, Chen J (2005) Regulation of p53-MDMX interaction by casein kinase 1 alpha. Mol Cell Biol 25, 6509-20
16024788   Curated Info