Ser358
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.4
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser358  -  MLKL (human)

Site Information
ELRkTQtsMSLGTtR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 22996309

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 9 ) , immunoassay ( 3 , 5 , 8 ) , immunoprecipitation ( 7 , 9 ) , mass spectrometry ( 9 ) , mutation of modification site ( 8 , 9 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 ) , western blotting ( 3 , 4 , 5 , 7 , 8 )
Disease tissue studied:
Alzheimer's disease ( 6 ) , breast cancer ( 3 ) , breast adenocarcinoma ( 3 ) , breast cancer, triple negative ( 3 ) , colorectal cancer ( 4 , 5 , 7 , 8 , 9 ) , colorectal carcinoma ( 4 , 5 , 7 , 8 , 9 ) , lymphoma ( 4 , 8 ) , fibrosarcoma of soft tissue ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
RIPK3 (human) ( 5 , 8 , 9 )
Kinases, in vitro:
RIPK3 (human) ( 9 )
Treatments:
adriamycin ( 3 ) , BV6 ( 3 ) , LPS ( 8 ) , Necrostatin-1 ( 8 ) , phenhydan ( 4 ) , resibufogenin ( 5 ) , siRNA ( 5 ) , TNF ( 3 , 4 , 8 ) , virus infection ( 3 ) , Z-VAD-FMK ( 3 , 4 , 8 )

Downstream Regulation
Effects of modification on MLKL:
intracellular localization ( 3 , 6 , 7 , 8 ) , molecular association, regulation ( 8 ) , protein conformation ( 6 , 7 )
Effects of modification on biological processes:
apoptosis, induced ( 3 , 8 ) , carcinogenesis, inhibited ( 5 ) , cell motility, inhibited ( 5 ) , signaling pathway regulation ( 9 ) , transcription, induced ( 5 )
Induce interaction with:
MLKL (human) ( 8 )

Disease / Diagnostics Relevance
Relevant diseases:
Alzheimer's disease ( 6 ) , colorectal cancer ( 5 )

References 

1

Chen X, et al. (2022) Mosaic composition of RIP1-RIP3 signalling hub and its role in regulating cell death. Nat Cell Biol
35256774   Curated Info

2

Zu R, et al. (2021) Quantitative analysis of phosphoproteome in necroptosis reveals a role of TRIM28 phosphorylation in promoting necroptosis-induced cytokine production. Cell Death Dis 12, 994
34689152   Curated Info

3

Zhang J, et al. (2021) Necroptotic virotherapy of oncolytic alphavirus M1 cooperated with Doxorubicin displays promising therapeutic efficacy in TNBC. Oncogene
34155344   Curated Info

4

Moerke C, et al. (2018) The anticonvulsive Phenhydan suppresses extrinsic cell death. Cell Death Differ
30442947   Curated Info

5

Han Q, et al. (2018) Resibufogenin suppresses colorectal cancer growth and metastasis through RIP3-mediated necroptosis. J Transl Med 16, 201
30029665   Curated Info

6

Caccamo A, et al. (2017) Necroptosis activation in Alzheimer's disease. Nat Neurosci 20, 1236-1246
28758999   Curated Info

7

Yoon S, Bogdanov K, Kovalenko A, Wallach D (2016) Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it. Cell Death Differ 23, 253-60
26184911   Curated Info

8

Wang H, et al. (2014) Mixed Lineage Kinase Domain-like Protein MLKL Causes Necrotic Membrane Disruption upon Phosphorylation by RIP3. Mol Cell 54, 133-46
24703947   Curated Info

9

Sun L, et al. (2012) Mixed lineage kinase domain-like protein mediates necrosis signaling downstream of RIP3 kinase. Cell 148, 213-27
22265413   Curated Info