Thr211

Site Information
QKDKFLQtFCGSPLY SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 456032
Available spectra: 2 CST

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 , 3 ) , mass spectrometry ( 1 , 5 ) , mutation of modification site ( 2 , 3 , 6 , 7 ) , peptide sequencing ( 7 ) , phospho-antibody ( 2 , 6 ) , western blotting ( 2 , 3 )
Disease tissue studied:	lung cancer ( 1 , 2 ) , non-small cell lung cancer ( 1 ) , non-small cell lung adenocarcinoma ( 1 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 2 ) , DLD1 (intestinal) ( 6 ) , DMS153 (pulmonary) ( 1 ) , HCT116 (intestinal) ( 6 ) , HeLa (cervical) [LKB1 (human)] ( 7 ) , lung ( 1 ) , MEF (fibroblast) [LKB1 (human)] ( 7 ) , MV4-11 (macrophage) ( 5 ) , NCI-H1437 (pulmonary) ( 1 ) , NCI-H2073 (pulmonary) ( 1 ) , NCI-H209 (pulmonary) ( 1 ) , NCI-H838 (pulmonary) ( 1 ) , SW480 (intestinal) ( 6 ) , WI-38 (fibroblast) ( 3 )

Downstream Regulation
Effects of modification on NuaK1:	enzymatic activity, induced ( 2 , 3 , 6 )
Effects of modification on biological processes:	cell cycle regulation ( 2 , 3 ) , cell growth, altered ( 3 , 6 ) , cell growth, inhibited ( 2 )

References
1	Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
2	Hou X, et al. (2011) A new role of NUAK1: directly phosphorylating p53 and regulating cell proliferation. Oncogene 30, 2933-42 21317932 Curated Info
3	Humbert N, et al. (2010) Regulation of ploidy and senescence by the AMPK-related kinase NUAK1. EMBO J 29, 376-86 19927127 Curated Info
4	Zagórska A, et al. (2010) New roles for the LKB1-NUAK pathway in controlling myosin phosphatase complexes and cell adhesion. Sci Signal 3, ra25 20354225 Curated Info
5	Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64 19369195 Curated Info
6	Suzuki A, Ogura T, Esumi H (2006) NDR2 acts as the upstream kinase of ARK5 during insulin-like growth factor-1 signaling. J Biol Chem 281, 13915-21 16488889 Curated Info
7	Lizcano JM, et al. (2004) LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J 23, 833-43 14976552 Curated Info