Thr179
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Home > Phosphorylation Site Page: > Thr179  -  C/EBP-beta (mouse)

Site Information
GSsGsLStssSssPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455887

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , electrophoretic mobility shift ( 4 ) , mass spectrometry ( 6 ) , mutation of modification site ( 6 ) , western blotting ( 4 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 6 ) , 3T3-L1 (fibroblast) ( 4 )

Upstream Regulation
Putative in vivo kinases:
GSK3B (mouse) ( 3 , 4 )
Kinases, in vitro:
ERK1 (human) ( 2 ) , GSK3A (human) ( 2 ) , GSK3B (human) ( 6 ) , GSK3B (mouse) ( 4 , 5 )
Treatments:
dexamethasone ( 4 ) , IBMX ( 4 ) , IL-17A ( 3 ) , insulin ( 4 ) , seliciclib ( 4 )

Downstream Regulation
Effects of modification on C/EBP-beta:
activity, induced ( 4 ) , molecular association, regulation ( 5 ) , protein conformation ( 5 )
Effects of modification on biological processes:
cell cycle regulation ( 4 ) , cell differentiation, altered ( 4 ) , transcription, altered ( 4 , 5 , 6 ) , transcription, inhibited ( 3 )
Induce interaction with:
C/EBP-beta (mouse) ( 5 ) , DNA ( 5 )

References 

1

Lee J, et al. (2012) Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts. J Biol Chem 287, 8839-51
22262865   Curated Info

2

Li X, et al. (2009) O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation. J Biol Chem 284, 19248-54
19478079   Curated Info

3

Shen F, et al. (2009) IL-17 receptor signaling inhibits C/EBPbeta by sequential phosphorylation of the regulatory 2 domain. Sci Signal 2, ra8
19244213   Curated Info

4

Li X, et al. (2007) Role of cdk2 in the sequential phosphorylation/activation of C/EBPbeta during adipocyte differentiation. Proc Natl Acad Sci U S A 104, 11597-602
17601773   Curated Info

5

Kim JW, Tang QQ, Li X, Lane MD (2007) Effect of phosphorylation and S-S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPbeta. Proc Natl Acad Sci U S A 104, 1800-4
17264204   Curated Info

6

Tang QQ, et al. (2005) Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis. Proc Natl Acad Sci U S A 102, 9766-71
15985551   Curated Info