Ser184
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Home > Phosphorylation Site Page: > Ser184  -  C/EBP-beta (mouse)

Site Information
LStssSssPPGtPSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455886

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , electrophoretic mobility shift ( 6 ) , mass spectrometry ( 1 , 3 , 4 , 9 ) , mutation of modification site ( 9 ) , western blotting ( 6 )
Relevant cell line - cell type - tissue:
3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 9 ) , 3T3-L1 (fibroblast) ( 6 ) , macrophage-peritoneum ( 1 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 4 )

Upstream Regulation
Putative in vivo kinases:
GSK3B (mouse) ( 6 )
Kinases, in vitro:
ERK1 (human) ( 5 ) , GSK3A (human) ( 5 ) , GSK3B (human) ( 9 ) , GSK3B (mouse) ( 6 , 7 )
Treatments:
dexamethasone ( 6 ) , IBMX ( 6 ) , insulin ( 6 ) , seliciclib ( 6 )

Downstream Regulation
Effects of modification on C/EBP-beta:
activity, induced ( 6 ) , molecular association, regulation ( 7 ) , protein conformation ( 7 )
Effects of modification on biological processes:
cell cycle regulation ( 6 ) , cell differentiation, altered ( 6 ) , transcription, altered ( 6 , 7 , 9 )
Induce interaction with:
C/EBP-beta (mouse) ( 7 ) , DNA ( 7 )

References 

1

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

2

Lee J, et al. (2012) Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts. J Biol Chem 287, 8839-51
22262865   Curated Info

3

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

4

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

5

Li X, et al. (2009) O-linked N-acetylglucosamine modification on CCAAT enhancer-binding protein beta: role during adipocyte differentiation. J Biol Chem 284, 19248-54
19478079   Curated Info

6

Li X, et al. (2007) Role of cdk2 in the sequential phosphorylation/activation of C/EBPbeta during adipocyte differentiation. Proc Natl Acad Sci U S A 104, 11597-602
17601773   Curated Info

7

Kim JW, Tang QQ, Li X, Lane MD (2007) Effect of phosphorylation and S-S bond-induced dimerization on DNA binding and transcriptional activation by C/EBPbeta. Proc Natl Acad Sci U S A 104, 1800-4
17264204   Curated Info

8

CeseƱa TI, Cardinaux JR, Kwok R, Schwartz J (2007) CCAAT/enhancer-binding protein (C/EBP) beta is acetylated at multiple lysines: acetylation of C/EBPbeta at lysine 39 modulates its ability to activate transcription. J Biol Chem 282, 956-67
17110376   Curated Info

9

Tang QQ, et al. (2005) Sequential phosphorylation of CCAAT enhancer-binding protein beta by MAPK and glycogen synthase kinase 3beta is required for adipogenesis. Proc Natl Acad Sci U S A 102, 9766-71
15985551   Curated Info