Lys386
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Home > Sumoylation Site Page: > Lys386  -  p53 (human)

Site Information
RHkkLMFktEGPDsD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 2255102

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 10 , 12 , 13 ) , mass spectrometry ( 1 , 2 ) , mutation of modification site ( 9 , 10 , 12 , 13 ) , western blotting ( 9 , 10 , 12 , 13 )
Disease tissue studied:
bone cancer ( 13 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
MDM2 (human) ( 12 ) , UBC9 (human) ( 9 )
Treatments:
actinomycin_D ( 12 ) , adriamycin ( 12 ) , calyculin_A ( 12 ) , H2O2 ( 10 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 13 )
Effects of modification on biological processes:
apoptosis, induced ( 12 ) , transcription, altered ( 10 , 13 )

References 

1

Hendriks IA, et al. (2014) Uncovering global SUMOylation signaling networks in a site-specific manner. Nat Struct Mol Biol 21, 927-36
25218447   Curated Info

2

Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2
24782567   Curated Info

3

Wang W, et al. (2014) PIASxα ligase enhances SUMO1 modification of PTEN protein as a SUMO E3 ligase. J Biol Chem 289, 3217-30
24344134   Curated Info

4

Hsiao HH, et al. (2009) "ChopNSpice," a mass spectrometric approach that allows identification of endogenous small ubiquitin-like modifier-conjugated peptides. Mol Cell Proteomics 8, 2664-75
19721078   Curated Info

5

Wu SY, Chiang CM (2009) p53 sumoylation: Mechanistic insights from reconstitution studies. Epigenetics 4, 445-451
19838051   Curated Info

6

Wu SY, Chiang CM (2009) Crosstalk between sumoylation and acetylation regulates p53-dependent chromatin transcription and DNA binding. EMBO J 28, 1246-59
19339993   Curated Info

7

Zimnik S, Gaestel M, Niedenthal R (2009) Mutually exclusive STAT1 modifications identified by Ubc9/substrate dimerization-dependent SUMOylation. Nucleic Acids Res 37, e30
19174562   Curated Info

8

Niedenthal R (2009) Enhanced detection of in vivo SUMO conjugation by Ubc9 fusion-dependent sumoylation (UFDS). Methods Mol Biol 497, 63-79
19107411   Curated Info

9

Jakobs A, et al. (2007) Ubc9 fusion-directed SUMOylation (UFDS): a method to analyze function of protein SUMOylation. Nat Methods 4, 245-50
17277783   Curated Info

10

Li T, et al. (2006) Expression of SUMO-2/3 induced senescence through p53- and pRB-mediated pathways. J Biol Chem 281, 36221-7
17012228   Curated Info

11

Schmidt D, Müller S (2002) Members of the PIAS family act as SUMO ligases for c-Jun and p53 and repress p53 activity. Proc Natl Acad Sci U S A 99, 2872-7
11867732   Curated Info

12

Muller S, et al. (2000) c-Jun and p53 activity is modulated by SUMO-1 modification. J Biol Chem 275, 13321-9
10788439   Curated Info

13

Gostissa M, et al. (1999) Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1. EMBO J 18, 6462-71
10562558   Curated Info