Ser424
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Home > Phosphorylation Site Page: > Ser424  -  HDAC3 (human)

Site Information
DHDNDKEsDVEI___   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455624

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 20 ) , immunoprecipitation ( 4 , 6 ) , mass spectrometry ( 2 , 5 , 8 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ) , modification-specific antibody ( 6 ) , mutation of modification site ( 20 ) , phospho-antibody ( 4 , 6 , 20 ) , phosphoamino acid analysis ( 20 ) , phosphopeptide mapping ( 20 ) , western blotting ( 4 , 6 , 20 )
Disease tissue studied:
breast cancer ( 4 , 5 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, triple negative ( 2 ) , cervical cancer ( 4 ) , cervical adenocarcinoma ( 4 ) , lymphoma ( 8 ) , Burkitt's lymphoma ( 8 ) , follicular lymphoma ( 8 ) , mantle cell lymphoma ( 8 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 20 ) , 293T (epithelial) ( 6 ) , A498 (renal) ( 14 ) , BJAB (B lymphocyte) ( 8 ) , breast ( 2 ) , BT-474 (breast cell) ( 5 ) , FL-318 (B lymphocyte) ( 8 ) , HeLa (cervical) ( 10 , 13 , 17 , 19 , 20 ) , HeLa S3 (cervical) ( 4 ) , JEKO-1 (B lymphocyte) ( 8 ) , Jurkat (T lymphocyte) ( 12 , 15 , 16 , 18 ) , K562 (erythroid) ( 10 ) , MCF-7 (breast cell) ( 4 , 5 ) , NCEB-1 (B lymphocyte) ( 8 ) , OCI-ly1 (B lymphocyte) ( 8 ) , Raji (B lymphocyte) ( 8 ) , RAMOS (B lymphocyte) ( 8 ) , SU-DHL-4 (B lymphocyte) ( 8 )

Upstream Regulation
Putative in vivo kinases:
CK2A1 (human) ( 4 )
Kinases, in vitro:
CK2A1 (human) ( 4 , 20 )
Putative upstream phosphatases:
PPP4C (human) ( 20 )
Treatments:
EGF ( 6 ) , insulin ( 6 )

Downstream Regulation
Effects of modification on HDAC3:
enzymatic activity, induced ( 4 , 20 )
Effects of modification on biological processes:
cell cycle regulation ( 4 )
Induce interaction with:
PKG1 (human) ( 6 )

References 

1

Han KA, et al. (2017) Leucine-rich repeat kinase 2 exacerbates neuronal cytotoxicity through phosphorylation of histone deacetylase 3 and histone deacetylation. Hum Mol Genet 26, 1-18
27798112   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

4

Patil H, et al. (2016) Mitotic Activation of a Novel Histone Deacetylase 3-Linker Histone H1.3 Protein Complex by Protein Kinase CK2. J Biol Chem 291, 3158-72
26663086   Curated Info

5

Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290
27362937   Curated Info

6

Wang S, et al. (2015) Insulin and mTOR Pathway Regulate HDAC3-Mediated Deacetylation and Activation of PGK1. PLoS Biol 13, e1002243
26356530   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42
24667141   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

11

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

12

Guo A (2011) CST Curation Set: 12456; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (K/R)Xp[ST](I/L/V)
Curated Info

13

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

14

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

15

Guo A (2010) CST Curation Set: 9182; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Guo A (2010) CST Curation Set: 9181; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8
19276368   Curated Info

18

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

19

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

20

Zhang X, et al. (2005) Histone deacetylase 3 (HDAC3) activity is regulated by interaction with protein serine/threonine phosphatase 4. Genes Dev 19, 827-39
15805470   Curated Info