Thr29
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Home > Phosphorylation Site Page: > Thr29  -  CDT1 (human)

Site Information
PPKLACRtPSPARPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455584

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 ) , mutation of modification site ( 12 ) , western blotting ( 7 )
Disease tissue studied:
breast cancer ( 4 ) , breast cancer, triple negative ( 4 )
Relevant cell line - cell type - tissue:
293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 9 ) , breast ( 4 ) , HEK293T (epithelial) ( 12 ) , HeLa (cervical) ( 3 , 7 , 8 , 11 , 12 ) , Jurkat (T lymphocyte) ( 5 , 10 ) , K562 (erythroid) ( 6 )

Upstream Regulation
Putative in vivo kinases:
JNK1 (human) ( 7 )
Kinases, in vitro:
CDK2 (human) ( 12 )
Treatments:
JNK_inhibitor_I ( 7 ) , sorbitol ( 7 ) , SP600125 ( 7 )

Downstream Regulation
Effects of modification on CDT1:
molecular association, regulation ( 12 )
Effects of modification on biological processes:
cell cycle regulation ( 12 ) , transcription, inhibited ( 7 )
Induce interaction with:
SKP2 (human) ( 12 )

References 

1

Zhou Y, et al. (2020) Distinct and sequential re-replication barriers ensure precise genome duplication. PLoS Genet 16, e1008988
32841231   Curated Info

2

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

3

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

6

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

7

Miotto B, Struhl K (2011) JNK1 phosphorylation of Cdt1 inhibits recruitment of HBO1 histone acetylase and blocks replication licensing in response to stress. Mol Cell 44, 62-71
21856198   Curated Info

8

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

9

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

10

Possemato A (2010) CST Curation Set: 9354; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]Pp[ST]
Curated Info

11

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

12

Takeda DY, Parvin JD, Dutta A (2005) Degradation of Cdt1 during S phase is Skp2-independent and is required for efficient progression of mammalian cells through S phase. J Biol Chem 280, 23416-23
15855168   Curated Info

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