Ser326

Site Information
ssVDtLLsPTALIDs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 455484

In vivo Characterization
Methods used to characterize site in vivo:	[32P] bio-synthetic labeling ( 55 ) , electrophoretic mobility shift ( 48 ) , immunoassay ( 5 , 37 , 41 , 44 ) , immunoprecipitation ( 14 , 22 , 29 , 36 , 39 , 46 ) , mass spectrometry ( 9 , 13 , 22 , 25 , 27 , 36 , 40 , 42 , 47 , 50 , 51 , 52 , 53 , 54 , 55 ) , mass spectrometry (in vitro) ( 4 , 9 , 49 ) , mutation of modification site ( 4 , 5 , 9 , 10 , 13 , 16 , 22 , 33 , 35 , 36 , 43 , 46 , 48 , 55 ) , peptide sequencing ( 55 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 21 , 22 , 23 , 24 , 26 , 28 , 29 , 30 , 31 , 32 , 34 , 35 , 36 , 37 , 38 , 43 , 44 , 45 , 48 , 49 , 55 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 24 , 26 , 28 , 29 , 30 , 31 , 32 , 34 , 35 , 36 , 37 , 38 , 39 , 43 , 44 , 45 , 46 , 48 , 49 )
Disease tissue studied:	bone cancer ( 12 , 30 ) , breast cancer ( 9 , 14 , 15 , 16 , 20 , 22 , 26 , 27 , 34 , 37 , 42 , 46 ) , breast adenocarcinoma ( 9 , 14 , 15 ) , breast ductal carcinoma ( 42 ) , HER2 positive breast cancer ( 25 ) , luminal A breast cancer ( 25 ) , luminal B breast cancer ( 25 ) , breast cancer, triple negative ( 9 , 14 , 15 , 25 ) , cervical cancer ( 50 ) , cervical adenocarcinoma ( 50 ) , colorectal cancer ( 18 , 32 ) , colorectal carcinoma ( 18 , 32 ) , endometrial cancer ( 19 ) , endometrial adenocarcinoma ( 19 ) , gallbladder cancer ( 8 ) , liver cancer ( 29 ) , lung cancer ( 21 , 26 , 31 , 43 , 44 ) , non-small cell lung cancer ( 21 , 26 , 31 , 43 , 44 ) , non-small cell lung adenocarcinoma ( 26 , 44 ) , non-small cell large cell lung carcinoma ( 21 , 31 , 43 ) , lymphoma ( 38 , 45 ) , B cell lymphoma ( 38 ) , non-Hodgkin's lymphoma ( 38 ) , mantle cell lymphoma ( 38 ) , ovarian cancer ( 19 , 28 , 34 , 42 ) , multiple myeloma ( 17 , 23 ) , prostate cancer ( 45 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 4 , 10 ) , A431 (epithelial) ( 22 ) , A549 (pulmonary) ( 26 , 44 ) , brain ( 39 ) , breast ( 25 , 37 , 42 ) , BT-20 (breast cell) ( 15 ) , BT-474 (breast cell) ( 15 , 16 , 20 , 26 , 37 ) , BT-549 (breast cell) ( 15 ) , CGL2 (fibroblast) ( 5 , 48 ) , epithelial-corneal ( 3 ) , GM05659 (fibroblast) ( 24 ) , GM18453 (fibroblast) ( 24 ) , HCT116 (intestinal) ( 18 , 32 ) , HEC-1B (endometrial) ( 19 ) , HEK293T (epithelial) ( 9 , 48 ) , HeLa (cervical) ( 6 , 30 , 33 , 40 , 46 , 48 , 49 , 51 , 54 , 55 ) , HeLa S3 (cervical) ( 50 ) , HepG2 (hepatic) ( 29 ) , HEY (ovarian) ( 28 ) , HFF1 (fibroblast) ( 44 ) , HTBoA (ovarian) ( 19 ) , IMR-90 (fibroblast) ( 21 ) , JEKO-1 (B lymphocyte) ( 38 ) , Jurkat (T lymphocyte) ( 47 , 52 ) , K562 (erythroid) ( 53 ) , KMS-18 (B lymphocyte) ( 23 ) , lymphocyte ( 10 ) , MCAS (ovarian) ( 19 ) , MCF-10A (breast cell) ( 34 ) , MCF-7 (breast cell) ( 14 , 15 , 16 , 20 , 27 , 34 , 37 , 46 ) , MCF10A1 (epithelial) ( 7 , 14 ) , MCF12A (epithelial) ( 14 ) , MDA-MB-231 (breast cell) ( 16 , 22 , 34 , 37 ) , MDA-MB-468 (breast cell) ( 9 , 14 , 15 ) , MEF (fibroblast) ( 13 , 33 , 43 ) , MM1.S (lymphoblast) ( 17 , 23 ) , monocyte-blood ( 2 , 41 ) , mononuclear-blood ( 2 ) , myoblast ( 10 ) , NCI-H460 (pulmonary) ( 21 , 31 , 43 ) , NCI-H929 (B lymphocyte) ( 17 ) , NOZ ('epithelial, gallbladder') ( 8 ) , ovary ( 42 ) , PC3 (prostate cell) ( 45 ) , RAW 264.7 (macrophage) ( 41 ) , rectal gland tubules ( 39 ) , RKO (intestinal) ( 32 ) , RPMI-8266 (plasma cell) ( 23 ) , SD1 (lymphoblastoid) ( 8 ) , SKBr3 (breast cell) ( 14 , 15 , 26 ) , SKOV-3 (ovarian) ( 28 , 34 ) , SMMC7721 (hepatocyte) ( 29 ) , T47D (breast cell) ( 14 , 15 ) , U-937 (myeloid) ( 45 ) , U266 (plasma cell) ( 17 , 23 ) , U2OS (bone cell) ( 12 , 30 ) , Z138 (B lymphocyte) ( 38 )

Upstream Regulation
Regulatory protein:	Akt2 (human) ( 4 ) , Akt3 (human) ( 4 ) , ER-alpha (human) ( 14 ) , HER2 (human) ( 37 ) , HRas (human) ( 7 ) , HSF2 (human) ( 12 ) , THBS4 (human) ( 8 )
Putative in vivo kinases:	Akt1 (human) ( 4 , 8 , 37 ) , DYRK2 (human) ( 4 , 6 , 9 ) , ERK1 (human) ( 35 ) , ERK2 (human) ( 35 ) , MEK1 (human) ( 4 , 36 ) , mTOR (human) ( 4 , 34 , 49 ) , P38A (human) ( 4 ) , P38G (human) ( 22 )
Kinases, in vitro:	Akt1 (human) ( 4 , 37 ) , Akt2 (human) ( 4 ) , DYRK2 (human) ( 4 , 9 ) , MEK1 (human) ( 4 , 36 ) , mTOR (human) ( 4 , 49 ) , P38A (human) ( 4 , 22 ) , P38B (human) ( 22 ) , P38D (human) ( 22 ) , P38G (human) ( 22 )
Treatments:	17-beta-estradiol ( 14 ) , 1Na-PP1 ( 9 ) , 1NM-PP1 ( 9 ) , 2,4-Bis(4-hydroxybenzyl)phenol ( 43 ) , 3-MB-PP1 ( 9 ) , 4-HT ( 14 ) , antibody ( 8 , 52 ) , arsenite ( 49 ) , As2O3 ( 5 , 48 ) , BIRB-0796 ( 22 ) , bisphenol_A ( 14 ) , bleomycin ( 21 ) , bortezomib ( 12 , 17 , 23 ) , carbendazim ( 32 ) , carfilzomib ( 17 ) , Cd(2+) ( 49 ) , celastrol ( 26 , 45 ) , Coniferyl aldehyde ( 35 ) , CPT ( 21 ) , cycloheximide ( 43 ) , ethanol ( 41 ) , faslodex ( 14 ) , fenbendazole ( 32 ) , formaldehyde ( 21 ) , geldanamycin ( 26 ) , glucose ( 29 ) , glucose_starvation ( 29 ) , harmine ( 9 ) , heat_shock ( 2 , 7 , 9 , 20 , 28 , 29 , 30 , 31 , 39 , 41 , 44 , 49 , 55 ) , heating ( 4 , 24 , 48 ) , heregulin ( 37 ) , hydroxyurea ( 21 ) , injury ( 3 ) , ischemia ( 42 ) , JNK_inhibitor_VIII ( 22 ) , KPT-185 ( 38 ) , KRIBB11 ( 7 ) , KU-0063794 ( 49 ) , lapatinib ( 20 , 37 ) , LPS ( 41 ) , LY294002 ( 8 , 14 , 37 , 48 ) , mebendazole ( 32 ) , MG132 ( 12 , 21 , 49 ) , MK-2206 ( 4 ) , mutation ( 39 ) , nocodazole ( 32 , 50 ) , novobiocin ( 45 ) , NU7026 ( 48 ) , NVP-AUY922 ( 15 ) , oxfendazole ( 32 ) , parbendazole ( 32 ) , PD98059 ( 48 ) , PEITC ( 22 ) , PI-103 ( 48 ) , piR-823 ( 18 ) , PPT ( 14 ) , rapamycin ( 4 , 9 , 14 , 29 , 48 , 49 ) , SB202190 ( 9 ) , SCH772984 ( 7 ) , selumetinib ( 7 , 36 ) , siRNA ( 4 , 9 , 34 , 37 , 49 ) , staurosporine ( 49 ) , tanespimycin ( 45 , 49 ) , taxol ( 5 ) , TG02 ( 17 ) , TPEN ( 2 ) , U0126 ( 14 , 22 , 32 , 35 , 36 ) , vinblastine ( 5 ) , virus infection ( 44 ) , X66 ( 26 ) , Zn(2+) ( 2 )

Downstream Regulation
Effects of modification on HSF1:	activity, induced ( 4 , 16 , 36 , 44 , 55 ) , enzymatic activity, induced ( 16 ) , intracellular localization ( 8 , 36 , 44 ) , molecular association, regulation ( 4 , 13 , 18 ) , phosphorylation ( 34 ) , protein conformation ( 22 ) , protein stabilization ( 7 , 9 , 20 , 35 , 36 , 43 )
Effects of modification on biological processes:	apoptosis, inhibited ( 2 , 9 , 48 ) , carcinogenesis, altered ( 29 ) , carcinogenesis, induced ( 7 , 8 , 9 , 37 ) , cell cycle regulation ( 5 ) , cell differentiation, induced ( 8 ) , cell growth, induced ( 3 , 5 , 8 , 16 ) , cell motility, induced ( 3 ) , cytoskeletal reorganization ( 5 ) , signaling pathway regulation ( 8 , 37 ) , transcription, altered ( 10 , 55 ) , transcription, induced ( 2 , 3 , 4 , 5 , 8 , 9 , 13 , 14 , 15 , 19 , 20 , 22 , 29 , 34 , 35 , 36 , 37 , 44 , 46 , 48 , 49 ) , transcription, inhibited ( 33 ) , translation, altered ( 10 ) , translation, induced ( 34 )
Induce interaction with:	CDK9 (human) ( 4 ) , GTF2B (human) ( 4 ) , PIN1 (human) ( 46 ) , RNA ( 18 ) , SGOL2 (human) ( 13 )

Disease / Diagnostics Relevance
Relevant diseases:	breast cancer ( 37 ) , acute myelogenous leukemia ( 10 ) , ovarian cancer ( 19 )

References
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