Ser292
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Home > Phosphorylation Site Page: > Ser292  -  HSF1 (human)

Site Information
DERPLSSsPLVRVkE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455481

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 2 , 3 , 6 , 7 , 8 ) , mutation of modification site ( 1 , 4 ) , peptide sequencing ( 8 ) , western blotting ( 4 )
Disease tissue studied:
breast cancer ( 4 ) , melanoma skin cancer ( 2 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 6 ) , HeLa (cervical) ( 1 , 4 , 8 ) , K562 (erythroid) ( 7 ) , liver ( 3 ) , MCF-7 (breast cell) ( 4 ) , MEF (fibroblast) ( 1 ) , WM239A (epidermal) ( 2 )

Downstream Regulation
Effects of modification on biological processes:
transcription, inhibited ( 1 )

References 

1

BudzyƄski MA, Puustinen MC, Joutsen J, Sistonen L (2015) Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 from Its Activation. Mol Cell Biol 35, 2530-40
25963659   Curated Info

2

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

3

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

4

Wang HY, Fu JC, Lee YC, Lu PJ (2013) Hyperthermia Stress Activates Heat Shock Protein Expression via Propyl Isomerase 1 Regulation with Heat Shock Factor 1. Mol Cell Biol 33, 4889-99
24126052   Curated Info

5

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

6

Brill LM, et al. (2009) Phosphoproteomic analysis of human embryonic stem cells. Cell Stem Cell 5, 204-13
19664994   Curated Info

7

Moritz A (2007) CST Curation Set: 2541; Year: 2007; Biosample/Treatment: cell line, K562/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

8

Guettouche T, Boellmann F, Lane WS, Voellmy R (2005) Analysis of phosphorylation of human heat shock factor 1 in cells experiencing a stress. BMC Biochem 6, 4
15760475   Curated Info