Ser231

Site Information
PEDAQKsssPAPADI SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 455387

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 )
Disease tissue studied:	acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 3 ) , acute megakaryoblastic leukemia (M7) ( 3 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 3 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 3 ) , acute myeloblastic leukemia, without maturation (M1) ( 3 ) , lung cancer ( 1 , 2 ) , non-small cell lung adenocarcinoma ( 1 , 2 ) , B cell lymphoma ( 3 ) , non-Hodgkin's lymphoma ( 3 ) , multiple myeloma ( 3 )
Relevant cell line - cell type - tissue:	'muscle, skeletal' ( 6 ) , 293 (epithelial) ( 8 ) , AML-193 (monocyte) ( 3 ) , Chang liver (cervical) ( 12 ) , CMK (megakaryoblast) ( 3 ) , CTS (myeloid) ( 3 ) , DOHH2 ('B lymphocyte, precursor') ( 3 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 4 ) , Flp-In T-Rex-293 (epithelial) ( 4 ) , HEK293T (epithelial) ( 10 , 14 ) , HEL (erythroid) ( 3 ) , HeLa (cervical) ( 9 , 11 , 13 ) , HUES-7 ('stem, embryonic') ( 7 ) , Jurkat (T lymphocyte) ( 15 , 16 ) , Kasumi-1 (myeloid) ( 3 ) , KG-1 (myeloid) ( 3 ) , lung ( 2 ) , MV4-11 (macrophage) ( 3 ) , OPM-2 (plasma cell) ( 3 ) , P31/FUJ (erythroid) ( 3 ) , PC9 (pulmonary) ( 1 ) , RL ('B lymphocyte, precursor') ( 3 ) , RPMI-8266 (plasma cell) ( 3 ) , SU-DHL-6 (B lymphocyte) ( 3 ) , U266 (plasma cell) ( 3 )

References
1	Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622 25814448 Curated Info
2	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
3	Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37 23628362 Curated Info
4	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
5	Rikova K (2011) CST Curation Set: 13116; Year: 2011; Biosample/Treatment: cell line, MGH-4/unknown; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info
6	Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65 19764811 Curated Info
7	Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26 19664995 Curated Info
8	Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501 19413330 Curated Info
9	Chen Y, et al. (2009) Combined integrin phosphoproteomic analyses and small interfering RNA--based functional screening identify key regulators for cancer cell adhesion and migration. Cancer Res 69, 3713-20 19351860 Curated Info
10	Tucker M (2009) CST Curation Set: 6216; Year: 2009; Biosample/Treatment: cell line, 293T/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
11	Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50 18452278 Curated Info
12	Sui S, et al. (2008) Phosphoproteome analysis of the human Chang liver cells using SCX and a complementary mass spectrometric strategy. Proteomics 8, 2024-34 18491316 Curated Info
13	Imami K, et al. (2008) Automated Phosphoproteome Analysis for Cultured Cancer Cells by Two-Dimensional NanoLC-MS Using a Calcined Titania/C18 Biphasic Column. Anal Sci 24, 161-6 18187866 Curated Info
14	Molina H, et al. (2007) Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci U S A 104, 2199-204 17287340 Curated Info
15	Zheng H, Hu P, Quinn DF, Wang YK (2005) Phosphotyrosine proteomic study of interferon alpha signaling pathway using a combination of immunoprecipitation and immobilized metal affinity chromatography. Mol Cell Proteomics 4, 721-30 15659558 Curated Info
16	Ficarro SB, et al. (2005) Automated immobilized metal affinity chromatography/nano-liquid chromatography/electrospray ionization mass spectrometry platform for profiling protein phosphorylation sites. Rapid Commun Mass Spectrom 19, 57-71 15570572 Curated Info