Lys827

Site Information
NsPNsILkAAkTLyE SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 21187456

In vivo Characterization
Methods used to characterize site in vivo:	flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , HEp-2 (squamous) ( 2 ) , Jurkat (T lymphocyte) ( 2 , 3 )

Downstream Regulation
Effects of modification on TTK:	protein degradation ( 1 )
Effects of modification on biological processes:	cell cycle regulation ( 1 )

References
1	Woodford MR, et al. (2021) Hsp90 chaperone code and the tumor suppressor VHL cooperatively regulate the mitotic checkpoint. Cell Stress Chaperones 26, 965-971 34586601 Curated Info
2	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
3	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
4	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info