Lys285

Site Information
WFkQDLPkYLFPEDP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 21128824

In vivo Characterization
Methods used to characterize site in vivo:	flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:	HEp-2 (squamous) ( 2 ) , hepatocyte-liver ( 1 ) , Jurkat (T lymphocyte) ( 2 ) , macrophage-bone marrow ( 1 )

Downstream Regulation
Effects of modification on AMPKA1:	enzymatic activity, inhibited ( 1 )

References
1	Zheng L, et al. (2020) Roquin-1 Regulates Macrophage Immune Response and Participates in Hepatic Ischemia-Reperfusion Injury. J Immunol 204, 1322-1333 31996460 Curated Info
2	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
3	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info