Thr290
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Home > Phosphorylation Site Page: > Thr290  -  Cot (human)

Site Information
FPKDLRGtEIYMSPE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455276

In vivo Characterization
Methods used to characterize site in vivo:
mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
LPS ( 3 ) , PS-1145 ( 1 ) , serum ( 1 )

Downstream Regulation
Effects of modification on Cot:
enzymatic activity, induced ( 1 , 3 ) , molecular association, regulation ( 2 ) , phosphorylation ( 1 ) , protein degradation ( 2 )
Inhibit interaction with:
NFkB-p105 (human) ( 2 )

References 

1

Stafford MJ, Morrice NA, Peggie MW, Cohen P (2006) Interleukin-1 stimulated activation of the COT catalytic subunit through the phosphorylation of Thr290 and Ser62. FEBS Lett 580, 4010-4
16806191   Curated Info

2

Cho J, Tsichlis PN (2005) Phosphorylation at Thr-290 regulates Tpl2 binding to NF-kappaB1/p105 and Tpl2 activation and degradation by lipopolysaccharide. Proc Natl Acad Sci U S A 102, 2350-5
15699325   Curated Info

3

Luciano BS, et al. (2004) Phosphorylation of threonine 290 in the activation loop of Tpl2/Cot is necessary but not sufficient for kinase activity. J Biol Chem 279, 52117-23
15466476   Curated Info