Ser366
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Home > Phosphorylation Site Page: > Ser366  -  p53 (human)

Site Information
PGGsRAHssHLkskk   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 455223

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 3 ) , mass spectrometry (in vitro) ( 5 ) , mutation of modification site ( 6 ) , phospho-antibody ( 1 , 3 , 6 ) , western blotting ( 1 , 2 , 3 , 6 )
Disease tissue studied:
bone cancer ( 2 , 6 ) , lung cancer ( 3 , 6 ) , non-small cell lung cancer ( 3 , 6 ) , prostate cancer ( 6 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 5 ) , HEK293T (epithelial) ( 1 , 2 , 6 ) , LNCaP (prostate cell) ( 6 ) , NCI-H1299 (pulmonary) ( 3 , 6 ) , Saos-2 (bone cell) ( 6 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Regulatory protein:
HIPK2 (human) ( 1 ) , PPP2CB (human) ( 2 )
Putative in vivo kinases:
Chk2 (human) ( 6 ) , Lmr1 (human) ( 1 )
Kinases, in vitro:
Chk2 (human) ( 6 ) , Lmr1 (human) ( 1 )
Putative upstream phosphatases:
PPP2CB (human) ( 2 )
Phosphatases, in vitro:
PPP2CB (human) ( 2 )
Treatments:
caffeine ( 6 ) , camptothecin ( 3 , 6 ) , ionizing_radiation ( 6 ) , siRNA ( 2 ) , UV ( 2 , 6 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 3 ) , molecular association, regulation ( 3 , 4 , 5 ) , protein stabilization ( 2 )
Effects of modification on biological processes:
carcinogenesis, inhibited ( 1 ) , cell growth, inhibited ( 1 ) , transcription, altered ( 6 ) , transcription, inhibited ( 1 )
Induce interaction with:
14-3-3 epsilon (human) ( 3 , 5 ) , 14-3-3 gamma (human) ( 3 , 5 ) , DNA ( 5 ) , p53 (human) ( 5 )
Inhibit interaction with:
S100A2 (human) ( 4 ) , S100B (human) ( 4 )

References 

1

Woods ML, et al. (2022) Epigenetically silenced apoptosis-associated tyrosine kinase (AATK) facilitates a decreased expression of Cyclin D1 and WEE1, phosphorylates TP53 and reduces cell proliferation in a kinase-dependent manner. Cancer Gene Ther
35902728   Curated Info

2

Park JH, Smith RJ, Shieh SY, Roeder RG (2011) The GAS41-PP2Cbeta complex dephosphorylates p53 at serine 366 and regulates its stability. J Biol Chem 286, 10911-7
21317290   Curated Info

3

Rajagopalan S, Sade RS, Townsley FM, Fersht AR (2010) Mechanistic differences in the transcriptional activation of p53 by 14-3-3 isoforms. Nucleic Acids Res 38, 893-906
19933256   Curated Info

4

van Dieck J, et al. (2009) Posttranslational modifications affect the interaction of S100 proteins with tumor suppressor p53. J Mol Biol 394, 922-30
19819244   Curated Info

5

Rajagopalan S, et al. (2008) 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers. Nucleic Acids Res 36, 5983-91
18812399   Curated Info

6

Ou YH, Chung PH, Sun TP, Shieh SY (2005) p53 C-terminal phosphorylation by CHK1 and CHK2 participates in the regulation of DNA-damage-induced C-terminal acetylation. Mol Biol Cell 16, 1684-95
15659650   Curated Info

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