Ser2

Site Information
______MsEAAPAAP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2048013

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 )
Disease tissue studied:	melanoma skin cancer ( 12 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 5 ) , 'brain, cerebellum' ( 13 ) , 'brain, cerebral cortex' ( 10 , 13 ) , 'brain, hippocampus, dentate gyrus' ( 13 ) , 'brain, midbrain' ( 13 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 11 ) , 32Dcl3 (myeloid) ( 11 ) , brain ( 9 ) , heart ( 6 ) , liver ( 1 ) , macrophage-bone marrow ( 8 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 8 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 7 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 7 ) , MEF (fibroblast) ( 7 ) , RAW 264.7 (macrophage) ( 3 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 12 )

Upstream Regulation
Regulatory protein:	FLT3 (mouse) ( 11 )

References
1	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
2	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
3	Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44 25367039 Curated Info
4	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
5	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
6	Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11 23737553 Curated Info
7	Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3 23429704 Curated Info
8	Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371 20531401 Curated Info
9	Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9 20415495 Curated Info
10	Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82 19737024 Curated Info
11	Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39 19854140 Curated Info
12	Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26 19367708 Curated Info
13	Trinidad JC, et al. (2008) Quantitative analysis of synaptic phosphorylation and protein expression. Mol Cell Proteomics 7, 684-96 18056256 Curated Info