Ser445

Site Information
TGVLLPssPEAEVPG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 2023457

In vivo Characterization
Methods used to characterize site in vivo:	flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	bone cancer ( 1 ) , lung cancer ( 2 ) , non-small cell lung cancer ( 2 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , HCC1359 (pulmonary) ( 2 ) , HCC2279 (pulmonary) ( 2 ) , HCC4006 (pulmonary) ( 2 ) , HCC827 (pulmonary) ( 2 ) , HeLa (cervical) ( 3 , 4 ) , LCLC-103H (pulmonary) ( 2 ) , LOU-NH91 (squamous) ( 2 ) , NCI-H1568 (pulmonary) ( 2 ) , NCI-H2172 (pulmonary) ( 2 ) , NCI-H460 (pulmonary) ( 2 ) , NCI-H647 (pulmonary) ( 2 ) , U2OS (bone cell) ( 1 )

Upstream Regulation
Putative in vivo kinases:	CDK1 (human) ( 1 )

References
1	Banerjee S, et al. (2014) Interplay between Polo kinase, LKB1-activated NUAK1 kinase, PP1βMYPT1 phosphatase complex and the SCFβTrCP E3 ubiquitin ligase. Biochem J 461, 233-45 24785407 Curated Info
2	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
3	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
4	Chen RQ, et al. (2009) CDC25B mediates rapamycin-induced oncogenic responses in cancer cells. Cancer Res 69, 2663-8 19276368 Curated Info