Thr2256
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Home > Phosphorylation Site Page: > Thr2256  -  NG2 (rat)

Site Information
YLRKRNKtGKHDVQV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454952

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 2 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 2 ) , phosphoamino acid analysis ( 2 ) , phosphopeptide mapping ( 2 ) , western blotting ( 1 , 2 )
Disease tissue studied:
brain cancer ( 1 , 2 ) , glioblastoma ( 1 , 2 ) , glioma ( 1 , 2 ) , melanoma skin cancer ( 1 )
Relevant cell line - cell type - tissue:
A375 (melanocyte) ( 1 ) , U251 (glial) ( 1 ) , U251 (glial) [NG2 (rat)] ( 2 )

Upstream Regulation
Putative in vivo kinases:
PKCA (human) ( 1 , 2 )
Kinases, in vitro:
PKCA (human) ( 2 )
Treatments:
GF109203X ( 2 ) , Go_6976 ( 1 , 2 ) , PDGF ( 1 ) , phorbol_ester ( 2 )

Downstream Regulation
Effects of modification on NG2:
intracellular localization ( 1 , 2 )
Effects of modification on biological processes:
cell adhesion, altered ( 2 ) , cell motility, altered ( 1 , 2 )

References 

1

Makagiansar IT, Williams S, Mustelin T, Stallcup WB (2007) Differential phosphorylation of NG2 proteoglycan by ERK and PKCalpha helps balance cell proliferation and migration. J Cell Biol 178, 155-65
17591920   Curated Info

2

Makagiansar IT, et al. (2004) Phosphorylation of NG2 proteoglycan by protein kinase C-alpha regulates polarized membrane distribution and cell motility. J Biol Chem 279, 55262-70
15504744   Curated Info