Ser399
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.6.0.2
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Ser399  -  CCNE1 (human)

Site Information
GLLTPPQsGKKQSSG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454414

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 7 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 6 ) , mutation of modification site ( 4 , 6 , 7 ) , peptide sequencing ( 6 , 7 ) , phospho-antibody ( 1 , 5 , 7 ) , phosphoamino acid analysis ( 7 ) , phosphopeptide mapping ( 7 ) , western blotting ( 1 , 5 , 7 )
Disease tissue studied:
bone cancer ( 7 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CDK1 (human) ( 1 ) , CDK2 (human) ( 5 , 7 )
Kinases, in vitro:
CDK2 (human) ( 7 )
Putative upstream phosphatases:
PPP2R5C (human) ( 1 )
Phosphatases, in vitro:
PPP2R5C (human) ( 1 )
Treatments:
calyculin_A ( 1 ) , seliciclib ( 1 )

Downstream Regulation
Effects of modification on CCNE1:
activity, induced ( 1 ) , intracellular localization ( 6 ) , molecular association, regulation ( 4 ) , protein degradation ( 1 , 4 , 6 , 7 )
Effects of modification on biological processes:
cell cycle regulation ( 1 )
Induce interaction with:
FBXW7 (human) ( 4 )

References 

1

Davis RJ, Swanger J, Hughes BT, Clurman BE (2017) The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation. Mol Cell Biol 37
28137908   Curated Info

2

Possemato A (2008) CST Curation Set: 5316; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

3

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

4

Hao B, et al. (2007) Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases. Mol Cell 26, 131-43
17434132   Curated Info

5

Geng Y, et al. (2007) Kinase-independent function of cyclin E. Mol Cell 25, 127-39
17218276   Curated Info

6

Ye X, et al. (2004) Recognition of phosphodegron motifs in human cyclin E by the SCF(Fbw7) ubiquitin ligase. J Biol Chem 279, 50110-9
15364936   Curated Info

7

Welcker M, et al. (2003) Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradation. Mol Cell 12, 381-92
14536078   Curated Info