Thr146

Site Information
KKEEGtGtLKKDRTL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 18639144

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 )
Disease tissue studied:	leukemia ( 3 )
Relevant cell line - cell type - tissue:	'3T3-L1, differentiated' (adipocyte) ( 1 ) , heart ( 2 ) , RAW 264.7 (macrophage) ( 3 )

Upstream Regulation
Treatments:	insulin ( 1 ) , MK-2206 ( 1 )

References
1	Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20 23684622 Curated Info
2	Deng N, et al. (2011) Phosphoproteome analysis reveals regulatory sites in major pathways of cardiac mitochondria. Mol Cell Proteomics 10, M110.000117 20495213 Curated Info
3	Possemato A (2009) CST Curation Set: 6932; Year: 2009; Biosample/Treatment: cell line, RAW 264.7/MG132; Disease: leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS/T) Immunoaffinity Beads Cat#: 1978 Curated Info