Tyr453
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Home > Phosphorylation Site Page: > Tyr453  -  SHPS1 (human)

Site Information
EPNNHTEyASIQTSP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1861200

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 , 5 , 6 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
gastric cancer ( 4 ) , leukemia ( 2 ) , chronic myelogenous leukemia ( 2 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
CTK (human) ( 1 )
Treatments:
IGF-1 ( 1 ) , LPS ( 5 ) , poly(I-C) ( 5 ) , PP2 ( 5 ) , siRNA ( 1 )

Downstream Regulation
Effects of modification on SHPS1:
molecular association, regulation ( 1 , 5 ) , phosphorylation ( 1 , 6 )
Effects of modification on biological processes:
cell growth, induced ( 1 ) , cell motility, induced ( 1 ) , signaling pathway regulation ( 1 , 5 )
Induce interaction with:
SHP-1 (human) ( 5 ) , SHP-2 (human) ( 1 , 5 )

References 

1

Radhakrishnan Y, et al. (2011) IGF-I Stimulates Cooperative Interaction between the IGF-I Receptor and CSK Homologous Kinase that Regulates SHPS-1 Phosphorylation in Vascular Smooth Muscle Cells. Mol Endocrinol 25, 1636-49
21799000   Curated Info

2

Possemato A (2010) CST Curation Set: 9954; Year: 2010; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

3

Jørgensen C, et al. (2009) Cell-specific information processing in segregating populations of Eph receptor ephrin-expressing cells. Science 326, 1502-9
20007894   Curated Info

4

Ren H (2008) CST Curation Set: 3791; Year: 2008; Biosample/Treatment: cell line, NUGC-4/serum starved; Disease: gastric cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Miyake A, et al. (2008) Negative regulation by SHPS-1 of Toll-like receptor-dependent proinflammatory cytokine production in macrophages. Genes Cells 13, 209-19
18233962   Curated Info

6

Stofega MR, et al. (2000) Negative regulation of growth hormone receptor/JAK2 signaling by signal regulatory protein alpha. J Biol Chem 275, 28222-9
10842184   Curated Info