Ser215
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Home > Phosphorylation Site Page: > Ser215  -  p53 (human)

Site Information
DrNtFrHsVVVPyEP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454242

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 ) , electrophoretic mobility shift ( 6 ) , immunoprecipitation ( 1 , 4 , 5 ) , mutation of modification site ( 1 , 2 , 3 , 4 , 5 , 6 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 4 , 5 )
Disease tissue studied:
bone cancer ( 4 ) , breast cancer ( 4 ) , liver cancer ( 1 ) , lung cancer ( 4 , 5 ) , non-small cell lung cancer ( 4 , 5 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
AurA (human) ( 6 ) , AurB (human) ( 4 ) , PAK4 (human) ( 1 )
Kinases, in vitro:
AurA (human) ( 3 , 6 ) , AurB (human) ( 4 ) , PAK4 (human) ( 1 )
Treatments:
AZD1152 ( 4 )

Downstream Regulation
Effects of modification on p53:
activity, inhibited ( 5 , 6 ) , molecular association, regulation ( 1 ) , protein conformation ( 5 ) , protein degradation ( 4 ) , protein stabilization ( 5 ) , ubiquitination ( 4 )
Effects of modification on biological processes:
apoptosis, inhibited ( 4 ) , carcinogenesis, induced ( 4 ) , cell cycle regulation ( 4 ) , cell growth, altered ( 2 ) , cell motility, inhibited ( 1 ) , transcription, altered ( 5 , 6 ) , transcription, induced ( 2 ) , transcription, inhibited ( 1 , 4 )
Inhibit interaction with:
DNA ( 1 )

References 

1

Xu HT, et al. (2016) PAK4 Phosphorylates p53 at Serine 215 to Promote Liver Cancer Metastasis. Cancer Res 76, 5732-5742
27496712   Curated Info

2

Jha HC, et al. (2015) EBNA3C regulates p53 through induction of Aurora kinase B. Oncotarget 6, 5788-803
25691063   Curated Info

3

Hsueh KW, et al. (2013) A novel Aurora-A-mediated phosphorylation of p53 inhibits its interaction with MDM2. Biochim Biophys Acta 1834, 508-515
23201157   Curated Info

4

Gully CP, et al. (2012) Aurora B kinase phosphorylates and instigates degradation of p53. Proc Natl Acad Sci U S A 109, E1513-22
22611192   Curated Info

5

Fraser JA, et al. (2010) A novel p53 phosphorylation site within the MDM2 ubiquitination signal: II. a model in which phosphorylation at SER269 induces a mutant conformation to p53. J Biol Chem 285, 37773-86
20847049   Curated Info

6

Liu Q, et al. (2004) Aurora-A abrogation of p53 DNA binding and transactivation activity by phosphorylation of serine 215. J Biol Chem 279, 52175-82
15469940   Curated Info