Ser389
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Home > Phosphorylation Site Page: > Ser389  -  p53 (mouse)

Site Information
VkkVGPDsD______   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447535

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 18 , 19 , 20 ) , electrophoretic mobility shift ( 13 ) , immunoprecipitation ( 7 , 17 ) , mass spectrometry ( 16 ) , mass spectrometry (in vitro) ( 16 ) , mutation of modification site ( 6 , 7 , 9 , 10 , 11 , 17 , 18 , 19 ) , phospho-antibody ( 3 , 7 , 8 , 9 , 10 , 11 , 13 , 14 , 16 ) , phosphoamino acid analysis ( 18 ) , phosphopeptide mapping ( 15 , 18 , 19 , 20 ) , western blotting ( 3 , 7 , 8 , 9 , 14 , 17 )
Disease tissue studied:
bladder cancer ( 9 ) , lung cancer ( 7 ) , non-small cell lung cancer ( 7 ) , lymphoma ( 9 ) , non-melanoma skin cancer ( 9 ) , testicular cancer ( 7 , 14 )
Relevant cell line - cell type - tissue:
'stem, embryonic' ( 8 ) , 3T3 (fibroblast) [p53 (mouse)] ( 15 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 13 , 15 ) , 3T3 (fibroblast) ( 17 ) , bladder ( 9 ) , COS (fibroblast) ( 19 ) , E.coli (bacterial) ( 16 ) , F9 (testicular) ( 7 , 14 ) , lymphocyte ( 9 ) , MEF (fibroblast) ( 6 , 9 ) , MEF (fibroblast) [IGF1R (mouse)] ( 10 , 11 ) , myocyte-heart ( 3 ) , NCI-H1299 (pulmonary) [p53 (human)] ( 7 ) , Rat1 (fibroblast) ( 18 ) , SF9 ( 16 ) , skin ( 9 ) , SV3T3 (fibroblast) ( 20 )

Upstream Regulation
Regulatory protein:
ATR (human) ( 5 ) , CCNE1 (human) ( 17 ) , HBpX (retrovirus) ( 5 ) , P38A (human) ( 5 )
Putative in vivo kinases:
CK2A1 (mouse) ( 15 )
Kinases, in vitro:
CK2A1 (mouse) ( 15 ) , CK2A1 (rat) ( 20 )
Treatments:
2-AAF ( 9 ) , DRB ( 15 ) , glucose ( 12 ) , hyperglycemia ( 3 ) , IGF-1 ( 12 ) , ionizing_radiation ( 9 ) , leptin ( 3 ) , UV ( 6 , 7 , 8 , 9 , 10 , 11 , 13 , 14 )

Downstream Regulation
Effects of modification on p53:
activity, induced ( 5 , 17 )
Effects of modification on biological processes:
apoptosis, altered ( 6 ) , apoptosis, induced ( 5 , 11 ) , cell cycle regulation ( 6 ) , cell growth, altered ( 6 , 9 ) , transcription, altered ( 6 , 10 , 11 ) , transcription, induced ( 5 , 17 )
Induce interaction with:
DNA ( 17 )

References 

1

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

2

Song L, et al. (2011) p85α mediates p53 K370 acetylation by p300 and regulates its promoter-specific transactivity in the cellular UVB response. Oncogene 30, 1360-71
21057544   Curated Info

3

Malhotra A, et al. (2009) Inhibition of p66ShcA redox activity in cardiac muscle cells attenuates hyperglycemia-induced oxidative stress and apoptosis. Am J Physiol Heart Circ Physiol 296, H380-8
19060130   Curated Info

4

Warnock LJ, et al. (2008) Influence of tetramerisation on site-specific post-translational modifications of p53: comparison of human and murine p53 tumor suppressor protein. Cancer Biol Ther 7, 1481-9
18769132   Curated Info

5

Wang WH, Hullinger RL, Andrisani OM (2008) Hepatitis B virus X protein via the p38MAPK pathway induces E2F1 release and ATR kinase activation mediating p53 apoptosis. J Biol Chem 283, 25455-67
18606816   Curated Info

6

Bruins W, et al. (2008) The absence of Ser389 phosphorylation in p53 affects the basal gene expression level of many p53-dependent genes and alters the biphasic response to UV exposure in mouse embryonic fibroblasts. Mol Cell Biol 28, 1974-87
18195040   Curated Info

7

Cecchinelli B, et al. (2006) Ser58 of mouse p53 is the homologue of human Ser46 and is phosphorylated by HIPK2 in apoptosis. Cell Death Differ 13, 1994-7
16729035   Curated Info

8

Feng L, et al. (2005) Functional analysis of the roles of posttranslational modifications at the p53 C terminus in regulating p53 stability and activity. Mol Cell Biol 25, 5389-95
15964796   Curated Info

9

Hoogervorst EM, et al. (2005) Lack of p53 Ser389 phosphorylation predisposes mice to develop 2-acetylaminofluorene-induced bladder tumors but not ionizing radiation-induced lymphomas. Cancer Res 65, 3610-6
15867355   Curated Info

10

Iwakuma T, et al. (2004) Mutation at p53 serine 389 does not rescue the embryonic lethality in mdm2 or mdm4 null mice. Oncogene 23, 7644-50
15361844   Curated Info

11

Bruins W, et al. (2004) Increased sensitivity to UV radiation in mice with a p53 point mutation at Ser389. Mol Cell Biol 24, 8884-94
15456863   Curated Info

12

Kang BP, et al. (2003) IGF-1 inhibits the mitochondrial apoptosis program in mesangial cells exposed to high glucose. Am J Physiol Renal Physiol 285, F1013-24
12876069   Curated Info

13

Parra M, et al. (2001) p53 Phosphorylation at serine 15 is required for transcriptional induction of the plasminogen activator inhibitor-1 (PAI-1) gene by the alkylating agent N-methyl-N'-nitro-N-nitrosoguanidine. J Biol Chem 276, 36303-10
11470783   Curated Info

14

Kapoor M, et al. (2000) Cooperative phosphorylation at multiple sites is required to activate p53 in response to UV radiation. Oncogene 19, 358-64
10656682   Curated Info

15

McKendrick L, Milne D, Meek D (1999) Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable. Mol Cell Biochem 191, 187-99
10094408   Curated Info

16

Otvos L, et al. (1998) A monoclonal antibody to a multiphosphorylated, conformational epitope at the carboxy-terminus of p53. Biochim Biophys Acta 1404, 457-74
9739174   Curated Info

17

Hao M, et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J Biol Chem 271, 29380-5
8910602   Curated Info

18

McKendrick L, Meek DW (1994) A novel system to investigate the phosphorylation of the p53 tumor suppressor protein by the protein kinase CK2. Cell Mol Biol Res 40, 555-61
7735330   Curated Info

19

Wang Y, Eckhart W (1992) Phosphorylation sites in the amino-terminal region of mouse p53. Proc Natl Acad Sci U S A 89, 4231-5
1584757   Curated Info

20

Meek DW, Simon S, Kikkawa U, Eckhart W (1990) The p53 tumour suppressor protein is phosphorylated at serine 389 by casein kinase II. EMBO J 9, 3253-60
2145148   Curated Info