Ser269
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Home > Phosphorylation Site Page: > Ser269  -  p53 (human)

Site Information
GNLLGRNsFEVRVCA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 17677100

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 3 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
bone cancer ( 1 ) , breast cancer ( 3 ) , colorectal cancer ( 3 ) , colorectal carcinoma ( 3 ) , lung cancer ( 1 , 2 ) , non-small cell lung cancer ( 1 , 2 ) , melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
AurB (human) ( 1 )
Kinases, in vitro:
AurB (human) ( 1 ) , DAPK1 (human) ( 3 )
Treatments:
ionizing_radiation ( 3 ) , UV ( 3 )

Downstream Regulation
Effects of modification on p53:
activity, inhibited ( 2 , 3 ) , intracellular localization ( 3 ) , protein conformation ( 2 ) , protein stabilization ( 2 ) , ubiquitination ( 2 )
Effects of modification on biological processes:
apoptosis, altered ( 1 ) , cell growth, altered ( 3 ) , transcription, altered ( 2 , 3 ) , transcription, inhibited ( 1 )
Induce interaction with:
HSP70 (human) ( 2 )
Inhibit interaction with:
DNA ( 2 )

References 

1

Wu L, Ma CA, Zhao Y, Jain A (2011) Aurora B interacts with NIR-p53, leading to p53 phosphorylation in its DNA-binding domain and subsequent functional suppression. J Biol Chem 286, 2236-44
20959462   Curated Info

2

Fraser JA, et al. (2010) A novel p53 phosphorylation site within the MDM2 ubiquitination signal: II. a model in which phosphorylation at SER269 induces a mutant conformation to p53. J Biol Chem 285, 37773-86
20847049   Curated Info

3

Fraser JA, Vojtesek B, Hupp TR (2010) A novel p53 phosphorylation site within the MDM2 ubiquitination signal: I. phosphorylation at SER269 in vivo is linked to inactivation of p53 function. J Biol Chem 285, 37762-72
20851891   Curated Info