Ser864
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Home > Phosphorylation Site Page: > Ser864  -  REST (human)

Site Information
TEDLsPPsPPLPKEN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453739

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 4 , 6 , 7 , 8 , 10 , 12 , 13 , 14 , 15 , 16 , 17 ) , mass spectrometry (in vitro) ( 1 ) , mutation of modification site ( 5 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Disease tissue studied:
adrenal cancer ( 5 ) , pheochromocytoma ( 5 ) , breast cancer ( 7 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 7 ) , cervical cancer ( 14 ) , cervical adenocarcinoma ( 14 ) , leukemia ( 12 ) , acute myelogenous leukemia ( 12 ) , ovarian cancer ( 7 ) , melanoma skin cancer ( 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
HRas (human) ( 5 )
Putative in vivo kinases:
ERK1 (human) ( 5 ) , ERK2 (human) ( 5 )
Kinases, in vitro:
ERK2 (human) ( 5 )
Putative upstream phosphatases:
CTDSP1 (human) ( 1 , 5 )
Phosphatases, in vitro:
CTDSP1 (human) ( 1 )
Treatments:
EGF ( 5 ) , PD184352 ( 5 )

Downstream Regulation
Effects of modification on REST:
molecular association, regulation ( 5 ) , protein conformation ( 1 ) , protein degradation ( 1 , 5 )
Effects of modification on biological processes:
cell differentiation, inhibited ( 5 ) , transcription, induced ( 1 )
Induce interaction with:
BTRC (human) ( 5 ) , PIN1 (human) ( 5 )
Inhibit interaction with:
RCOR1 (human) ( 5 )

References 

1

Burkholder NT, et al. (2018) Phosphatase activity of Small C-terminal domain phosphatase 1 (SCP1) controls the stability of the key neuronal regulator RE1-silencing transcription factor (REST). J Biol Chem
30217818   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

4

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

5

Nesti E, et al. (2014) C-terminal domain small phosphatase 1 and MAP kinase reciprocally control REST stability and neuronal differentiation. Proc Natl Acad Sci U S A 111, E3929-36
25197063   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

9

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

10

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

11

Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25
22424773   Curated Info

12

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

13

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

14

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

15

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

16

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

17

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info