Thr94
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Home > Phosphorylation Site Page: > Thr94  -  PLCL1 (mouse)

Site Information
KCGGRKKtVsFSSMP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453436

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 )
Disease tissue studied:
melanoma skin cancer ( 3 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 'brain, embryonic' ( 4 ) , brain ( 5 ) , COS (fibroblast) ( 6 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 3 )

Upstream Regulation
Kinases, in vitro:
PKACA (mouse) ( 6 )
Treatments:
colforsin ( 6 ) , insulin ( 2 ) , LY294002 ( 2 ) , MK-2206 ( 2 )

Downstream Regulation
Effects of modification on PLCL1:
molecular association, regulation ( 6 )
Inhibit interaction with:
PPP1CA (mouse) ( 6 )

References 

1

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

2

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

3

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

4

Possemato A (2008) CST Curation Set: 3827; Year: 2008; Biosample/Treatment: tissue, brain, embryonic/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKA Substrate Antibody Cat#: 9621
Curated Info

5

Possemato A (2008) CST Curation Set: 3841; Year: 2008; Biosample/Treatment: tissue, brain/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

6

Terunuma M, et al. (2004) GABAA receptor phospho-dependent modulation is regulated by phospholipase C-related inactive protein type 1, a novel protein phosphatase 1 anchoring protein. J Neurosci 24, 7074-84
15306641   Curated Info