Ser696
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Home > Phosphorylation Site Page: > Ser696  -  MYPT1 (rat)

Site Information
QARQSRRstQGVTLT   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451998

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 3 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 7 ) , western blotting ( 2 , 4 , 5 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 7 ) , 'muscle, smooth'-'heart, artery' ( 2 ) , 'muscle, smooth'-thoracic aorta ( 6 ) , cerebral artery ( 5 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 3 ) , PC-12 (chromaffin) ( 3 ) , VSMC-aorta ( 4 )

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 4 ) , PKG1 (human) ( 4 )
Kinases, in vitro:
PKACA (cow) ( 1 )
Treatments:
cGMP_analog ( 5 , 7 ) , colforsin ( 2 ) , H-89 ( 4 ) , KT5823 ( 4 ) , L-NAME ( 5 ) , microcystin-LR ( 2 ) , NaF ( 6 ) , pioglitazone ( 4 ) , Triton_X-100 ( 5 ) , U46619 ( 2 , 5 ) , Y27632 ( 6 )

References 

1

Sutherland C, MacDonald JA, Walsh MP (2016) Analysis of phosphorylation of the myosin-targeting subunit of myosin light chain phosphatase by Phos-tag SDS-PAGE. Am J Physiol Cell Physiol 310, C681-91
26864694   Curated Info

2

Grassie ME, et al. (2012) Cross-talk between Rho-associated Kinase and Cyclic Nucleotide-dependent Kinase Signaling Pathways in the Regulation of Smooth Muscle Myosin Light Chain Phosphatase. J Biol Chem 287, 36356-69
22948155   Curated Info

3

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

4

Atkins KB, Irey B, Xiang N, Brosius FC (2009) A rapid, PPAR-{gamma}-dependent effect of pioglitazone on the phosphorylation of MYPT. Am J Physiol Cell Physiol 296, C1151-61
19261909   Curated Info

5

Neppl RL, et al. (2009) Thromboxane A2-induced bi-directional regulation of cerebral arterial tone. J Biol Chem 284, 6348-60
19095646   Curated Info

6

Jeon SB, et al. (2006) A role for Rho kinase in vascular contraction evoked by sodium fluoride. Biochem Biophys Res Commun 343, 27-33
16527249   Curated Info

7

Wooldridge AA, et al. (2004) Smooth muscle phosphatase is regulated in vivo by exclusion of phosphorylation of threonine 696 of MYPT1 by phosphorylation of Serine 695 in response to cyclic nucleotides. J Biol Chem 279, 34496-504
15194681   Curated Info