Ser22
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Home > Phosphorylation Site Page: > Ser22  -  RXRA (mouse)

Site Information
VNSSSLNsPTGRGSM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453093

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , electrophoretic mobility shift ( 9 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 ) , mutation of modification site ( 1 , 8 , 9 ) , phospho-antibody ( 1 ) , phosphopeptide mapping ( 9 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 3 , 5 ) , 'fat, brown' ( 7 ) , adipocyte-adipose tissue ( 1 ) , adipose tissue ( 1 ) , COS (fibroblast) ( 9 ) , kidney ( 7 ) , liver ( 2 , 4 , 6 , 7 ) , liver [leptin (mouse), homozygous knockout] ( 6 ) , preadipocyte ( 1 )

Upstream Regulation
Regulatory protein:
IRS1 (mouse) ( 1 ) , IRS2 (mouse) ( 1 )
Treatments:
high-fat diet ( 1 ) , insulin ( 1 , 5 ) , LY294002 ( 5 ) , MK-2206 ( 1 ) , refeeding ( 1 ) , U0126 ( 1 )

Downstream Regulation
Effects of modification on biological processes:
cell cycle regulation ( 8 ) , cell growth, altered ( 8 ) , transcription, altered ( 8 )

References 

1

Ardenkjær-Larsen J, et al. (2020) Insulin-induced serine 22 phosphorylation of retinoid X receptor alpha is dispensable for adipogenesis in brown adipocytes. Adipocyte 9, 142-152
32249683   Curated Info

2

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

3

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

4

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

5

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

6

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

7

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

8

Bastien J, et al. (2002) The phosphorylation site located in the A region of retinoic X receptor alpha is required for the antiproliferative effect of retinoic acid (RA) and the activation of RA target genes in F9 cells. J Biol Chem 277, 28683-9
12032153   Curated Info

9

Adam-Stitah S, Penna L, Chambon P, Rochette-Egly C (1999) Hyperphosphorylation of the retinoid X receptor alpha by activated c-Jun NH2-terminal kinases. J Biol Chem 274, 18932-41
10383391   Curated Info