Ser101
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Home > Phosphorylation Site Page: > Ser101  -  RhoGDI alpha (human)

Site Information
LEsFKkQsFVLkEGV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452195

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 10 ) , [32P] bio-synthetic labeling ( 10 ) , immunoprecipitation ( 3 ) , mass spectrometry ( 2 , 5 , 6 , 7 ) , mutation of modification site ( 1 , 3 , 9 , 10 ) , phospho-antibody ( 3 , 9 ) , western blotting ( 3 , 9 )
Disease tissue studied:
breast cancer ( 3 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 ) , lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 6 ) , pancreatic cancer ( 9 ) , pancreatic carcinoma ( 9 )
Relevant cell line - cell type - tissue:
breast ( 2 ) , HEK293T (epithelial) [PAK1 (human)] ( 10 ) , HEK293T (epithelial) [RhoGDI alpha (human)] ( 10 ) , HUVEC (endothelial) ( 1 ) , Jurkat (T lymphocyte) ( 7 ) , liver ( 5 ) , lung ( 6 ) , MCF-7 (breast cell) ( 3 ) , MIN6 (pancreatic) ( 9 )

Upstream Regulation
Putative in vivo kinases:
PAK1 (human) ( 10 )
Kinases, in vitro:
PAK1 (human) ( 10 )
Treatments:
glucose ( 9 )

Downstream Regulation
Effects of modification on RhoGDI alpha:
enzymatic activity, inhibited ( 1 ) , molecular association, regulation ( 3 , 9 ) , phosphorylation ( 3 )
Induce interaction with:
RhoGDI alpha (human) ( 3 )
Inhibit interaction with:
CDC42 iso1 (human) ( 9 ) , RAC1 (human) ( 9 )

References 

1

Xie F, et al. (2020) Differential phosphorylation regulates the shear stress-induced polar activity of Rho-specific guanine nucleotide dissociation inhibitor α. J Cell Physiol 235
32003021   Curated Info

2

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

3

Xiao Y, et al. (2014) 14-3-3τ promotes breast cancer invasion and metastasis by inhibiting RhoGDIα. Mol Cell Biol 34, 2635-49
24820414   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

6

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

7

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

8

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

9

Wang Z, Thurmond DC (2010) Differential phosphorylation of RhoGDI mediates the distinct cycling of Cdc42 and Rac1 to regulate second-phase insulin secretion. J Biol Chem 285, 6186-97
20028975   Curated Info

10

DerMardirossian C, Schnelzer A, Bokoch GM (2004) Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase. Mol Cell 15, 117-27
15225553   Curated Info

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