Ser516
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Home > Phosphorylation Site Page: > Ser516  -  AR (human)

Site Information
VSRVPYPsPTCVkSE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452166

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 12 ) , electrophoretic mobility shift ( 11 ) , immunoassay ( 8 ) , immunoprecipitation ( 3 , 5 ) , mass spectrometry ( 6 , 7 , 12 ) , mutation of modification site ( 5 , 10 , 11 , 12 ) , phospho-antibody ( 2 , 4 , 5 , 8 , 11 ) , western blotting ( 2 , 3 , 5 , 8 , 11 )
Disease tissue studied:
breast cancer ( 7 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 6 ) , luminal A breast cancer ( 6 ) , luminal B breast cancer ( 6 ) , breast cancer, surrounding tissue ( 6 ) , breast cancer, triple negative ( 6 ) , endometrial cancer ( 11 ) , endometrial adenocarcinoma ( 11 ) , prostate cancer ( 3 , 5 , 8 , 11 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
KEPI (human) ( 3 ) , XPB (human) ( 5 )
Putative in vivo kinases:
CDK1 (human) ( 8 ) , ERK1 (human) ( 11 ) , ERK2 (human) ( 11 )
Kinases, in vitro:
CDK7 (human) ( 10 )
Treatments:
androstanolone ( 8 ) , EGF ( 11 ) , ischemia ( 7 ) , metribolone ( 12 ) , seliciclib ( 8 ) , tripolide ( 5 ) , U0126 ( 11 )

Downstream Regulation
Effects of modification on AR:
intracellular localization ( 3 ) , protein degradation ( 10 ) , ubiquitination ( 10 )
Effects of modification on biological processes:
cell cycle regulation ( 3 ) , cell growth, induced ( 8 ) , transcription, altered ( 10 ) , transcription, induced ( 5 , 11 )

Disease / Diagnostics Relevance
Relevant diseases:
breast ductal carcinoma ( 4 ) , breast cancer, triple negative ( 4 ) , prostate cancer ( 8 )

References 

1

Nicoll JX, Fry AC, Mosier EM (2021) Androgen and glucocorticoid receptor phosphorylation following resistance exercise and pre-workout supplementation. Steroids 172, 108859
33974920   Curated Info

2

Nicoll JX, Fry AC, Mosier EM (2019) Sex-based differences in resting MAPK, androgen, and glucocorticoid receptor phosphorylation in human skeletal muscle. Steroids 141, 23-29
30414425   Curated Info

3

Grey J, et al. (2018) Differential regulation of the androgen receptor by protein phosphatase regulatory subunits. Oncotarget 9, 3922-3935
29423094   Curated Info

4

Roseweir AK, et al. (2017) Phosphorylation of androgen receptors at serine 515 is a potential prognostic marker for triple negative breast cancer. Oncotarget 8, 37172-37185
28415597   Curated Info

5

Han Y, et al. (2017) Triptolide Inhibits the AR Signaling Pathway to Suppress the Proliferation of Enzalutamide Resistant Prostate Cancer Cells. Theranostics 7, 1914-1927
28638477   Curated Info

6

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Willder JM, et al. (2013) Androgen receptor phosphorylation at serine 515 by Cdk1 predicts biochemical relapse in prostate cancer patients. Br J Cancer 108, 139-48
23321516   Curated Info

9

Chen S, Gulla S, Cai C, Balk SP (2012) Androgen receptor serine 81 phosphorylation mediates chromatin binding and transcriptional activation. J Biol Chem 287, 8571-83
22275373   Curated Info

10

Chymkowitch P, et al. (2011) The phosphorylation of the androgen receptor by TFIIH directs the ubiquitin/proteasome process. EMBO J 30, 468-79
21157430   Curated Info

11

Ponguta LA, Gregory CW, French FS, Wilson EM (2008) Site-specific androgen receptor serine phosphorylation linked to epidermal growth factor-dependent growth of castration-recurrent prostate cancer. J Biol Chem 283, 20989-1001
18511414   Curated Info

12

Wong HY, et al. (2004) Phosphorylation of androgen receptor isoforms. Biochem J 383, 267-76
15239671   Curated Info