Lys224

Site Information
NDkIVIEkQGkGTAD SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15489469

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 4 , 5 , 6 , 7 , 8 )
Disease tissue studied:	lung cancer ( 4 ) , non-small cell lung adenocarcinoma ( 4 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 4 ) , HEp-2 (squamous) ( 1 ) , Jurkat (T lymphocyte) ( 1 , 5 , 6 ) , U2OS (bone cell) ( 7 )

Upstream Regulation
Treatments:	vorinostat ( 4 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Lumpkin RJ, et al. (2017) Site-specific identification and quantitation of endogenous SUMO modifications under native conditions. Nat Commun 8, 1171 29079793 Curated Info
3	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
4	Wu Q, et al. (2015) Suberoylanilide hydroxamic acid treatment reveals crosstalks among proteome, ubiquitylome and acetylome in non-small cell lung cancer A549 cell line. Sci Rep 5, 9520 25825284 Curated Info
5	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
6	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
7	Povlsen LK, et al. (2012) Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. Nat Cell Biol 14, 1089-98 23000965 Curated Info
8	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info