Lys166

Site Information
ESHQFQPkNFWNGRW SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15385891

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 )
Disease tissue studied:	leukemia ( 3 ) , T cell leukemia ( 3 )
Relevant cell line - cell type - tissue:	HEK293T (epithelial) ( 2 ) , Jurkat (T lymphocyte) ( 1 , 3 ) , MV4-11 (macrophage) ( 2 )

References
1	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
2	Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284 21890473 Curated Info
3	Guo A (2010) CST Curation Set: 9184; Year: 2010; Biosample/Treatment: cell line, Jurkat/untreated; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info