Arg174
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Home > Mono-methylation Site Page: > Arg174  -  CSDA (human)

Site Information
GSrYAADrrrYRrGY   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 15380241
Available spectra:  6 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 )
Disease tissue studied:
colorectal cancer ( 12 ) , colorectal carcinoma ( 12 ) , gastric cancer ( 9 ) , lung cancer ( 4 , 5 , 6 ) , non-small cell lung cancer ( 4 , 5 , 6 , 7 , 8 , 10 , 11 ) , non-small cell lung adenocarcinoma ( 4 , 5 , 6 ) , non-small cell large cell lung carcinoma ( 5 , 6 ) , non-small cell squamous cell lung carcinoma ( 11 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , 293T (epithelial) ( 3 ) , 293T (epithelial) [PADI4 (human), no information, doxycyline inducible over-expression of PADI4] ( 3 ) , A549 (pulmonary) ( 4 , 8 ) , HCC78 (pulmonary) ( 6 ) , HCT116 (intestinal) ( 12 ) , Jurkat (T lymphocyte) ( 2 ) , lung ( 4 , 5 , 6 ) , MKN-45 (gastric) ( 9 ) , NCI-H1299 (pulmonary) ( 5 ) , NCI-H1355 (pulmonary) ( 4 ) , NCI-H1703 (squamous) ( 11 ) , NCI-H1734 (pulmonary) ( 5 ) , NCI-H1781 (pulmonary) ( 6 ) , NCI-H1792 (pulmonary) ( 4 ) , NCI-H1944 (pulmonary) ( 5 ) , NCI-H2228 (pulmonary) ( 6 ) , NCI-H23 (pulmonary) ( 4 ) , NCI-H3122 (pulmonary) ( 6 , 7 ) , NCI-H3255 (pulmonary) ( 10 ) , NCI-H358 (pulmonary) ( 5 ) , NCI-H441 (pulmonary) ( 4 ) , NCI-H460 (pulmonary) ( 5 ) , NCI-H661 (pulmonary) ( 6 ) , U2OS (bone cell) ( 3 )

References 

1

Larsen SC, et al. (2016) Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells. Sci Signal 9, rs9
27577262   Curated Info

2

Geoghegan V, et al. (2015) Comprehensive identification of arginine methylation in primary T cells reveals regulatory roles in cell signalling. Nat Commun 6, 6758
25849564   Curated Info

3

Sylvestersen KB, et al. (2014) Proteomic analysis of arginine methylation sites in human cells reveals dynamic regulation during transcriptional arrest. Mol Cell Proteomics 13, 2072-88
24563534   Curated Info

4

Rikova K (2013) CST Curation Set: 20096; Year: 2013; Biosample/Treatment: cell line, A549, H1355, H23, H441, H1792; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

5

Rikova K (2013) CST Curation Set: 20097; Year: 2013; Biosample/Treatment: cell line, H1299, H1944, H358, H1734, H460; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

6

Rikova K (2013) CST Curation Set: 20100; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

7

Rikova K (2013) CST Curation Set: 18851; Year: 2013; Biosample/Treatment: cell line, H3122/crizotinib, geldanamycin; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

8

Rikova K (2012) CST Curation Set: 16037; Year: 2012; Biosample/Treatment: cell line, A549/iressa, gleevec, crizotinib; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine (Me-R4-100) Rabbit mAb Cat#: 8015
Curated Info

9

Rikova K (2012) CST Curation Set: 16034; Year: 2012; Biosample/Treatment: cell line, MKN45/crizotinib; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

10

Rikova K (2012) CST Curation Set: 16035; Year: 2012; Biosample/Treatment: cell line, H3255/iressa; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

11

Rikova K (2012) CST Curation Set: 16036; Year: 2012; Biosample/Treatment: cell line, H1703/gleevec; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine [mme-R] MultiMab(TM) Rabbit mAb mix Cat#: 8015
Curated Info

12

Possemato A (2010) CST Curation Set: 9753; Year: 2010; Biosample/Treatment: cell line, HCT 116/control; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m1R Antibodies Used to Purify Peptides prior to LCMS: Mono-Methyl Arginine (Me-R(4)-100) Rabbit mAb Cat#: 8015
Curated Info