Thr852
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Home > Phosphorylation Site Page: > Thr852  -  MYPT1 (mouse)

Site Information
PREKRRstGVSFWtQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452075

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 3 , 4 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Relevant cell line - cell type - tissue:
'muscle, smooth' ( 1 ) , 'stem, embryonic' ( 3 , 4 ) , small mesenteric arteries ( 1 ) , stomach ( 2 )

Upstream Regulation
Regulatory protein:
ROCK2 (human) ( 1 ) , RSK2 (human) ( 1 )
Kinases, in vitro:
ROCK1 (human) ( 5 ) , ROCK2 (human) ( 1 )
Treatments:
angiotensin_2 ( 1 ) , blood pressure ( 1 ) , carbachol ( 2 ) , electrical_stimulation ( 2 ) , endothelin ( 1 ) , KCl ( 2 ) , phenylephrine ( 1 ) , U46619 ( 1 )

Downstream Regulation
Effects of modification on MYPT1:
enzymatic activity, inhibited ( 1 )

References 

1

Artamonov MV, et al. (2018) RSK2 contributes to myogenic vasoconstriction of resistance arteries by activating smooth muscle myosin and the Na/H exchanger. Sci Signal 11
30377223   Curated Info

2

An C, et al. (2015) Role of Telokin in Regulating Murine Gastric Fundus Smooth Muscle Tension. PLoS One 10, e0134876
26258553   Curated Info

3

Tucker M (2009) CST Curation Set: 8559; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

4

Tucker M (2009) CST Curation Set: 8557; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS/T) (110B7) Rabbit mAb Cat#: 9614, PTMScan(R) Phospho-Akt Substrate Motif (RXXS*/T*) Immunoaffinity Beads Cat#: 1978
Curated Info

5

Feng J, et al. (1999) Inhibitory phosphorylation site for Rho-associated kinase on smooth muscle myosin phosphatase. J Biol Chem 274, 37385-90
10601309   Curated Info