Thr855
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Home > Phosphorylation Site Page: > Thr855  -  MYPT1 (rat)

Site Information
PREKRRstGVSFWTQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452075

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 6 , 7 ) , phospho-antibody ( 2 , 4 , 8 , 9 , 10 , 11 , 12 , 13 , 14 ) , western blotting ( 2 , 4 , 5 , 8 , 9 , 10 , 12 , 14 )
Disease tissue studied:
adrenal cancer ( 5 , 6 , 7 ) , pheochromocytoma ( 5 , 6 , 7 )
Relevant cell line - cell type - tissue:
'muscle, smooth'-'heart, artery' ( 4 , 13 ) , cerebral artery ( 9 ) , PC-12 (chromaffin) ( 5 , 6 , 7 ) , PC12D (chromaffin) ( 11 ) , pulmonary artery ( 10 ) , vascular smooth muscle cell ('muscle, smooth') ( 12 ) , VSMC-aorta ( 8 , 14 )

Upstream Regulation
Regulatory protein:
PIK3C2A (rat) ( 12 ) , RHOA (human) ( 14 ) , SMN (rat) ( 5 )
Kinases, in vitro:
PKACA (cow) ( 1 ) , ROCK1 (rat) ( 1 )
Treatments:
BAPTA-AM ( 12 ) , cGMP_analog ( 9 ) , colforsin ( 4 ) , depolarization ( 3 ) , dinoprost ( 10 ) , hypoxia ( 10 ) , ionomycin ( 12 ) , L-NAME ( 9 ) , LPA ( 11 ) , microcystin-LR ( 4 ) , norepinephrine ( 12 ) , pioglitazone ( 8 ) , salicylate ( 3 ) , SU6656 ( 10 ) , U46619 ( 2 , 4 , 9 , 13 ) , Y27632 ( 2 , 8 , 10 , 11 , 12 , 13 )

Downstream Regulation
Effects of modification on MYPT1:
activity, inhibited ( 9 ) , intracellular localization ( 9 )

References 

1

Sutherland C, MacDonald JA, Walsh MP (2016) Analysis of phosphorylation of the myosin-targeting subunit of myosin light chain phosphatase by Phos-tag SDS-PAGE. Am J Physiol Cell Physiol 310, C681-91
26864694   Curated Info

2

Reddi BA, et al. (2015) Attenuated platelet aggregation in patients with septic shock is independent from the activity state of myosin light chain phosphorylation or a reduction in Rho kinase-dependent inhibition of myosin light chain phosphatase. Intensive Care Med Exp 3, 37
26215804   Curated Info

3

Mills RD, et al. (2015) A Role for the Tyrosine Kinase Pyk2 in Depolarization-induced Contraction of Vascular Smooth Muscle. J Biol Chem 290, 8677-92
25713079   Curated Info

4

Grassie ME, et al. (2012) Cross-talk between Rho-associated Kinase and Cyclic Nucleotide-dependent Kinase Signaling Pathways in the Regulation of Smooth Muscle Myosin Light Chain Phosphatase. J Biol Chem 287, 36356-69
22948155   Curated Info

5

Nölle A, et al. (2011) The spinal muscular atrophy disease protein SMN is linked to the Rho-kinase pathway via profilin. Hum Mol Genet 20, 4865-78
21920940   Curated Info

6

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

7

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

8

Atkins KB, Irey B, Xiang N, Brosius FC (2009) A rapid, PPAR-{gamma}-dependent effect of pioglitazone on the phosphorylation of MYPT. Am J Physiol Cell Physiol 296, C1151-61
19261909   Curated Info

9

Neppl RL, et al. (2009) Thromboxane A2-induced bi-directional regulation of cerebral arterial tone. J Biol Chem 284, 6348-60
19095646   Curated Info

10

Knock GA, et al. (2008) Role of src-family kinases in hypoxic vasoconstriction of rat pulmonary artery. Cardiovasc Res 80, 453-62
18682436   Curated Info

11

Takefuji M, et al. (2007) Rho-kinase modulates the function of STEF, a Rac GEF, through its phosphorylation. Biochem Biophys Res Commun 355, 788-94
17320046   Curated Info

12

Yoshioka K, Sugimoto N, Takuwa N, Takuwa Y (2007) Essential role for class II phosphoinositide 3-kinase alpha-isoform in Ca2+-induced, Rho- and Rho kinase-dependent regulation of myosin phosphatase and contraction in isolated vascular smooth muscle cells. Mol Pharmacol 71, 912-20
17179444   Curated Info

13

Wilson DP, et al. (2005) Thromboxane A2-induced contraction of rat caudal arterial smooth muscle involves activation of Ca2+ entry and Ca2+ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697. Biochem J 389, 763-74
15823093   Curated Info

14

Pang H, et al. (2005) RhoA-Rho kinase pathway mediates thrombin- and U-46619-induced phosphorylation of a myosin phosphatase inhibitor, CPI-17, in vascular smooth muscle cells. Am J Physiol Cell Physiol 289, C352-60
15814590   Curated Info