Ser303

Site Information
QATYFPPsPPSSEPG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 14652801

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 2 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 , 2 ) , western blotting ( 1 , 2 )
Disease tissue studied:	breast cancer ( 2 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) ( 1 ) , B5/589 (epithelial) ( 2 ) , DLD1 (intestinal) ( 1 ) , HCT116 (intestinal) ( 1 ) , HEK293T (epithelial) ( 2 ) , MCF-10A (breast cell) ( 2 ) , MCF-7 (breast cell) ( 2 )

Downstream Regulation
Effects of modification on KLF5:	molecular association, regulation ( 1 , 2 ) , protein degradation ( 1 , 2 ) , ubiquitination ( 2 )
Effects of modification on biological processes:	carcinogenesis, altered ( 2 ) , transcription, inhibited ( 1 )
Induce interaction with:	FBXW7 (human) ( 1 , 2 )

References
1	Bialkowska AB, Liu Y, Nandan MO, Yang VW (2014) A colon cancer-derived mutant of Krüppel-like factor 5 (KLF5) is resistant to degradation by glycogen synthase kinase 3β (GSK3β) and the E3 ubiquitin ligase F-box and WD repeat domain-containing 7α (FBW7α). J Biol Chem 289, 5997-6005 24398687 Curated Info
2	Zhao D, Zheng HQ, Zhou Z, Chen C (2010) The Fbw7 tumor suppressor targets KLF5 for ubiquitin-mediated degradation and suppresses breast cell proliferation. Cancer Res 70, 4728-38 20484041 Curated Info