Ser137
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Home > Phosphorylation Site Page: > Ser137  -  DARPP-32 (rat)

Site Information
EEDEEEDsQAEVLKG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448046

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 3 , 4 ) , mass spectrometry ( 1 ) , mutation of modification site ( 4 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
'brain, brainstem' ( 1 ) , 'brain, cerebral cortex' ( 1 ) , 'brain, striatum' ( 4 ) , 'brain, substantia nigra' ( 4 ) , nerve ( 3 ) , stomach ( 1 )

Upstream Regulation
Kinases, in vitro:
CK1A (human) ( 3 , 4 ) , PKACA (cow) ( 5 )
Putative upstream phosphatases:
PPM1B (human) ( 3 )
Phosphatases, in vitro:
PPM1B (human) ( 3 ) , PPP2CA (human) ( 3 ) , PPP3CA (human) ( 5 )
Treatments:
cAMP_analog ( 3 , 5 ) , levamfetamine ( 2 ) , okadaic_acid ( 3 ) , PF-670462 ( 2 )

Downstream Regulation
Effects of modification on DARPP-32:
phosphorylation ( 5 )
Effects of modification on biological processes:
cell motility, induced ( 2 ) , signaling pathway regulation ( 2 )

References 

1

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

2

Li D, et al. (2011) Casein kinase 1 enables nucleus accumbens amphetamine-induced locomotion by regulating AMPA receptor phosphorylation. J Neurochem 118, 237-47
21564097   Curated Info

3

Desdouits F, et al. (1998) Dephosphorylation of Ser-137 in DARPP-32 by protein phosphatases 2A and 2C: different roles in vitro and in striatonigral neurons. Biochem J 330 ( Pt 1), 211-6
9461512   Curated Info

4

Desdouits F, et al. (1995) Phosphorylation of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein, by casein kinase I in vitro and in vivo. J Biol Chem 270, 8772-8
7721783   Curated Info

5

Desdouits F, Siciliano JC, Greengard P, Girault JA (1995) Dopamine- and cAMP-regulated phosphoprotein DARPP-32: phosphorylation of Ser-137 by casein kinase I inhibits dephosphorylation of Thr-34 by calcineurin. Proc Natl Acad Sci U S A 92, 2682-5
7708705   Curated Info