Thr75
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Home > Phosphorylation Site Page: > Thr75  -  DARPP-32 (rat)

Site Information
RPNPCAYtPPSLKAV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448048

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 1 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 ) , western blotting ( 1 , 2 , 5 )
Relevant cell line - cell type - tissue:
'brain, caudate-putamen' ( 4 , 5 ) , 'brain, nucleus accumbens' ( 4 , 5 ) , 'brain, striatum' ( 1 , 3 ) , 'neuron, cortical' ( 2 ) , 'neuron, striatal'-brain ( 1 ) , COS (fibroblast) ( 1 ) , Neuro-2a (neuron) ( 2 )

Upstream Regulation
Treatments:
acetyl-L-carnitine ( 5 ) , AMPA ( 2 ) , BAPTA-AM ( 2 ) , cocaine ( 4 , 5 ) , dopamine_depletion ( 3 ) , ionomycin ( 2 ) , levodopa ( 3 ) , morphine ( 4 ) , NMDA ( 2 )

Downstream Regulation
Effects of modification on biological processes:
transcription, altered ( 5 )

References 

1

Engmann O, et al. (2015) DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nat Commun 6, 10099
26639316   Curated Info

2

Ahn JH, et al. (2007) The B''/PR72 subunit mediates Ca2+-dependent dephosphorylation of DARPP-32 by protein phosphatase 2A. Proc Natl Acad Sci U S A 104, 9876-81
17535922   Curated Info

3

Brown AM, Deutch AY, Colbran RJ (2005) Dopamine depletion alters phosphorylation of striatal proteins in a model of Parkinsonism. Eur J Neurosci 22, 247-56
16029214   Curated Info

4

Scheggi S, et al. (2004) Dopamine and cyclic AMP-regulated phosphoprotein-32 phosphorylation pattern in cocaine and morphine-sensitized rats. J Neurochem 90, 792-9
15287884   Curated Info

5

Scheggi S, et al. (2004) Repeated acetyl-l-carnitine administration increases phospho-Thr34 DARPP-32 levels and antagonizes cocaine-induced increase in Cdk5 and phospho-Thr75 DARPP-32 levels in rat striatum. Eur J Neurosci 19, 1609-20
15066157   Curated Info