Thr34
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Home > Phosphorylation Site Page: > Thr34  -  DARPP-32 (rat)

Site Information
MIRRRRPtPALLFRV   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448047

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 12 ) , immunoprecipitation ( 2 , 5 ) , mass spectrometry ( 2 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 15 ) , western blotting ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 10 , 15 )
Disease tissue studied:
breast cancer ( 5 )
Relevant cell line - cell type - tissue:
'brain, caudate-putamen' ( 9 , 10 ) , 'brain, hippocampus' ( 8 ) , 'brain, nucleus accumbens' ( 6 , 9 , 10 , 11 ) , 'brain, striatum' ( 2 , 3 , 15 ) , 'neuron, cerebellar granule'-brain ( 15 ) , 'neuron, dorsal horn'-spinal cord ( 1 ) , 'neuron, striatal'-brain ( 2 ) , COS (fibroblast) ( 2 ) , MCF-7 (breast cell) ( 5 ) , nerve ( 12 ) , neuron-'brain, hippocampus' ( 7 )

Upstream Regulation
Kinases, in vitro:
PKACA (cow) ( 13 ) , PKACA (human) ( 14 ) , PKACA (mouse) ( 15 )
Phosphatases, in vitro:
PPP1CA (rabbit) ( 7 ) , PPP3CA (human) ( 7 , 13 )
Treatments:
5-HT ( 15 ) , 8-Rp-cAMP ( 13 ) , acetyl-L-carnitine ( 10 ) , ANP ( 15 ) , APV ( 8 ) , APV_withdrawal ( 7 ) , benserazide ( 3 ) , cAMP_analog ( 12 ) , cGMP_analog ( 15 ) , cocaine ( 6 , 10 ) , colforsin ( 15 ) , dopamine ( 15 ) , ethanol ( 11 ) , GBR 12935 ( 1 ) , isoproterenol ( 15 ) , KN-93 ( 8 ) , levamfetamine ( 4 ) , levodopa ( 3 ) , long-term_potentiation ( 8 ) , morphine ( 9 ) , okadaic_acid ( 12 ) , PF-670462 ( 4 ) , propranolol ( 15 ) , Ro25-6981 ( 1 ) , SCH_23390 ( 11 ) , SKF38393 ( 11 ) , VIP ( 15 )

Downstream Regulation
Effects of modification on DARPP-32:
enzymatic activity, inhibited ( 4 ) , phosphorylation ( 4 ) , protein conformation ( 14 )
Effects of modification on biological processes:
cell motility, induced ( 4 ) , signaling pathway regulation ( 4 ) , transcription, altered ( 10 )
Induce interaction with:
PPP1CA (human) ( 5 )

References 

1

Buesa I, Aira Z, Azkue JJ (2016) Regulation of Nociceptive Plasticity Threshold and DARPP-32 Phosphorylation in Spinal Dorsal Horn Neurons by Convergent Dopamine and Glutamate Inputs. PLoS One 11, e0162416
27610622   Curated Info

2

Engmann O, et al. (2015) DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons. Nat Commun 6, 10099
26639316   Curated Info

3

Ren T, et al. (2011) Sustained-release formulation of levodopa methyl ester/benserazide for prolonged suppressing dyskinesia expression in 6-OHDA-leisoned rats. Neurosci Lett 502, 117-22
21835223   Curated Info

4

Li D, et al. (2011) Casein kinase 1 enables nucleus accumbens amphetamine-induced locomotion by regulating AMPA receptor phosphorylation. J Neurochem 118, 237-47
21564097   Curated Info

5

Hansen C, et al. (2009) Wnt-5a-induced phosphorylation of DARPP-32 inhibits breast cancer cell migration in a CREB-dependent manner. J Biol Chem 284, 27533-43
19651774   Curated Info

6

Zhou L, et al. (2009) Basal and cocaine-induced sex differences in the DARPP-32-mediated signaling pathway. Psychopharmacology (Berl) 203, 175-83
18985320   Curated Info

7

Chung HJ, et al. (2009) Neuronal activity regulates phosphorylation-dependent surface delivery of G protein-activated inwardly rectifying potassium channels. Proc Natl Acad Sci U S A 106, 629-34
19118198   Curated Info

8

Atkins CM, et al. (2005) Bidirectional regulation of cytoplasmic polyadenylation element-binding protein phosphorylation by Ca2+/calmodulin-dependent protein kinase II and protein phosphatase 1 during hippocampal long-term potentiation. J Neurosci 25, 5604-10
15944388   Curated Info

9

Scheggi S, et al. (2004) Dopamine and cyclic AMP-regulated phosphoprotein-32 phosphorylation pattern in cocaine and morphine-sensitized rats. J Neurochem 90, 792-9
15287884   Curated Info

10

Scheggi S, et al. (2004) Repeated acetyl-l-carnitine administration increases phospho-Thr34 DARPP-32 levels and antagonizes cocaine-induced increase in Cdk5 and phospho-Thr75 DARPP-32 levels in rat striatum. Eur J Neurosci 19, 1609-20
15066157   Curated Info

11

Maldve RE, et al. (2002) DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nat Neurosci 5, 641-8
12068305   Curated Info

12

Desdouits F, et al. (1998) Dephosphorylation of Ser-137 in DARPP-32 by protein phosphatases 2A and 2C: different roles in vitro and in striatonigral neurons. Biochem J 330 ( Pt 1), 211-6
9461512   Curated Info

13

Desdouits F, Siciliano JC, Greengard P, Girault JA (1995) Dopamine- and cAMP-regulated phosphoprotein DARPP-32: phosphorylation of Ser-137 by casein kinase I inhibits dephosphorylation of Thr-34 by calcineurin. Proc Natl Acad Sci U S A 92, 2682-5
7708705   Curated Info

14

Neyroz P, et al. (1993) Study of the conformation of DARPP-32, a dopamine- and cAMP-regulated phosphoprotein, by fluorescence spectroscopy. J Biol Chem 268, 24022-31
8226946   Curated Info

15

Snyder GL, et al. (1992) Phosphorylation of DARPP-32 and protein phosphatase inhibitor-1 in rat choroid plexus: regulation by factors other than dopamine. J Neurosci 12, 3071-83
1494946   Curated Info