Ser11

Site Information
KRIAKRRsPPADAIP SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 452007

In vivo Characterization
Methods used to characterize site in vivo:	2D analysis ( 50 ) , electrophoretic mobility shift ( 50 ) , mass spectrometry ( 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 51 , 52 , 53 , 55 , 56 , 57 , 58 ) , mutation of modification site ( 1 , 50 , 54 , 59 ) , phospho-antibody ( 1 , 54 , 59 ) , western blotting ( 1 , 54 )
Disease tissue studied:	breast cancer ( 6 , 12 , 13 , 25 , 26 ) , breast ductal carcinoma ( 12 ) , HER2 positive breast cancer ( 4 ) , luminal A breast cancer ( 4 ) , luminal B breast cancer ( 4 ) , breast cancer, surrounding tissue ( 4 ) , breast cancer, triple negative ( 4 , 12 ) , cervical cancer ( 1 , 46 ) , cervical adenocarcinoma ( 46 ) , cervical squamous cell carcinoma ( 1 ) , leukemia ( 27 , 48 ) , acute myelogenous leukemia ( 27 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 24 ) , acute megakaryoblastic leukemia (M7) ( 24 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 24 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 24 ) , chronic myelogenous leukemia ( 48 ) , lung cancer ( 10 , 18 , 26 , 34 ) , non-small cell lung cancer ( 26 ) , non-small cell lung adenocarcinoma ( 10 , 18 , 34 ) , lymphoma ( 14 ) , Burkitt's lymphoma ( 14 ) , follicular lymphoma ( 14 ) , mantle cell lymphoma ( 14 ) , neuroblastoma ( 23 ) , ovarian cancer ( 12 ) , pancreatic ductal adenocarcinoma ( 17 ) , melanoma skin cancer ( 9 )
Relevant cell line - cell type - tissue:	'pancreatic, ductal'-pancreas ( 17 ) , 293 (epithelial) [ADRB1 (human), no information, overexpresses human beta1-adrenergic (ß1AR- HEK293)] ( 52 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 44 ) , 786-O (renal) [VHL (human), transfection] ( 7 ) , A498 (renal) ( 45 ) , A549 (pulmonary) ( 19 ) , AML-193 (monocyte) ( 24 ) , BJAB (B lymphocyte) ( 14 ) , breast ( 4 , 12 ) , BT-549 (breast cell) ( 26 ) , CMK (megakaryoblast) ( 24 ) , COS (fibroblast) ( 55 ) , endothelial-aorta ( 28 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 30 ) , H2077 (pulmonary) ( 26 ) , H322M (pulmonary) ( 26 ) , HCC1937 (breast cell) ( 26 ) , HCC366 (pulmonary) ( 26 ) , HCC78 (pulmonary) ( 26 ) , HCC827 (pulmonary) ( 26 ) , HEK293T (epithelial) ( 8 , 56 ) , HEL (erythroid) ( 24 ) , HeLa (cervical) [OGT (rat), transfection] ( 47 ) , HeLa (cervical) ( 1 , 3 , 11 , 22 , 32 , 37 , 39 , 41 , 47 , 49 , 51 , 52 , 53 , 54 , 57 , 58 , 59 ) , HeLa S3 (cervical) ( 46 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 13 ) , HMLER ('stem, breast cancer') ( 13 ) , HOP62 (pulmonary) ( 26 ) , HUES-9 ('stem, embryonic') ( 36 ) , JEKO-1 (B lymphocyte) ( 14 ) , Jurkat (T lymphocyte) ( 20 , 33 , 35 , 38 , 40 , 42 , 43 ) , K562 (erythroid) ( 22 , 48 ) , Kasumi-1 (myeloid) ( 24 ) , keratinocyte-skin ( 1 ) , KG-1 (myeloid) ( 24 ) , LCLC-103H (pulmonary) ( 26 ) , liver ( 16 ) , LOU-NH91 (squamous) ( 26 ) , lung ( 18 ) , MCF-7 (breast cell) ( 6 , 26 ) , MDA-MB-231 (breast cell) ( 26 ) , MV4-11 (macrophage) ( 24 , 27 ) , NB10 (neural crest) ( 23 ) , NCEB-1 (B lymphocyte) ( 14 ) , NCI-H1395 (pulmonary) ( 26 ) , NCI-H1568 (pulmonary) ( 26 ) , NCI-H157 (pulmonary) ( 26 ) , NCI-H1666 (pulmonary) ( 26 ) , NCI-H2030 (pulmonary) ( 26 ) , NCI-H322 (pulmonary) ( 26 ) , NCI-H460 (pulmonary) ( 26 ) , NCI-H520 (squamous) ( 26 ) , NCI-H647 (pulmonary) ( 26 ) , NPC (neural crest) ( 23 ) , OCI-ly1 (B lymphocyte) ( 14 ) , ovary ( 12 ) , P31/FUJ (erythroid) ( 24 , 27 ) , PC9 (pulmonary) ( 10 , 26 ) , PC9-IR (pulmonary) ( 10 ) , Raji (B lymphocyte) ( 14 ) , RAMOS (B lymphocyte) ( 14 ) , RPE-1 (retinal) ( 1 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 15 ) , SH-SY5Y (neural crest) ( 15 ) , SiHa (squamous) ( 1 ) , SKBr3 (breast cell) ( 25 ) , SU-DHL-4 (B lymphocyte) ( 14 ) , T lymphocyte-blood ( 29 ) , tsBN2 (fibroblast) ( 50 , 54 ) , U-1810 (pulmonary) [EFNB3 (human), knockdown] ( 34 ) , U-1810 (pulmonary) ( 34 ) , UPN-1 (B lymphocyte) ( 14 ) , Vero E6-S ('epithelial, kidney') ( 2 ) , WM239A (melanocyte) ( 9 )

Upstream Regulation
Regulatory protein:	Akt1 (human) ( 1 ) , CDK1 (human) ( 1 ) , E7 (papillomavirus) ( 1 ) , HPV16 protein E6 (papillomavirus) ( 1 )
Kinases, in vitro:	CDK1 (human) ( 50 )
Treatments:	antibody ( 29 ) , EGF ( 3 ) , LRRK2-IN-1 ( 15 ) , LY294002 ( 1 ) , MK-2206 ( 1 ) , rapamycin ( 1 )

Downstream Regulation
Effects of modification on RCC1:	intracellular localization ( 54 , 59 ) , molecular association, regulation ( 50 )
Effects of modification on biological processes:	cell cycle regulation ( 1 ) , cell growth, altered ( 54 ) , cytoskeletal reorganization ( 50 )
Inhibit interaction with:	KPNA4 (human) ( 50 )

References
1	Hou X, et al. (2021) Phosphorylation of RCC1 on Serine 11 Facilitates G1/S Transition in HPV E7-Expressing Cells. Biomolecules 11 34356619 Curated Info
2	Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell 182 32645325 Curated Info
3	Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296 27281782 Curated Info
4	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
5	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
6	Carrier M, et al. (2016) Phosphoproteome and Transcriptome of RA-Responsive and RA-Resistant Breast Cancer Cell Lines. PLoS One 11, e0157290 27362937 Curated Info
7	Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72 26506913 Curated Info
8	Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23 25278378 Curated Info
9	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
10	Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622 25814448 Curated Info
11	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
12	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
13	Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90 24782546 Curated Info
14	Rolland D, et al. (2014) Global phosphoproteomic profiling reveals distinct signatures in B-cell non-Hodgkin lymphomas. Am J Pathol 184, 1331-42 24667141 Curated Info
15	Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76 24117733 Curated Info
16	Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62 24275569 Curated Info
17	Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948 24670416 Curated Info
18	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
19	Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9 23836654 Curated Info
20	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
21	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
22	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
23	DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513 24349301 Curated Info
24	Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37 23628362 Curated Info
25	Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57 22964224 Curated Info
26	Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68 22617229 Curated Info
27	Alcolea MP, et al. (2012) Phosphoproteomic analysis of leukemia cells under basal and drug-treated conditions identifies markers of kinase pathway activation and mechanisms of resistance. Mol Cell Proteomics 11, 453-66 22547687 Curated Info
28	Verano-Braga T, et al. (2012) Time-resolved quantitative phosphoproteomics: new insights into Angiotensin-(1-7) signaling networks in human endothelial cells. J Proteome Res 11, 3370-81 22497526 Curated Info
29	Ruperez P, Gago-Martinez A, Burlingame AL, Oses-Prieto JA (2012) Quantitative phosphoproteomic analysis reveals a role for serine and threonine kinases in the cytoskeletal reorganization in early T cell receptor activation in human primary T cells. Mol Cell Proteomics 11, 171-86 22499768 Curated Info
30	Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70 22496350 Curated Info
31	Beli P, et al. (2012) Proteomic Investigations Reveal a Role for RNA Processing Factor THRAP3 in the DNA Damage Response. Mol Cell 46, 212-25 22424773 Curated Info
32	Grosstessner-Hain K, et al. (2011) Quantitative phospho-proteomics to investigate the polo-like kinase 1-dependent phospho-proteome. Mol Cell Proteomics 10, M111.008540 21857030 Curated Info
33	Guo A (2011) CST Curation Set: 12741; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
34	Ståhl S, et al. (2011) Phosphoproteomic profiling of NSCLC cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. J Proteome Res 10, 2566-78 21413766 Curated Info
35	Guo A (2011) CST Curation Set: 11425; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
36	Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3 21406692 Curated Info
37	Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5 21712546 Curated Info
38	Possemato A (2010) CST Curation Set: 10837; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
39	Zhou J (2010) CST Curation Set: 10708; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
40	Possemato A (2010) CST Curation Set: 10720; Year: 2010; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
41	Zhou J (2010) CST Curation Set: 10684; Year: 2010; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
42	Possemato A (2010) CST Curation Set: 10053; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
43	Possemato A (2010) CST Curation Set: 10051; Year: 2010; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
44	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
45	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
46	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
47	Wang Z, et al. (2010) Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal 3, ra2 20068230 Curated Info
48	Moritz A (2009) CST Curation Set: 8719; Year: 2009; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: PXpSP, pSPX(K/R) Antibodies Used to Purify Peptides prior to LCMS: Phospho-MAPK/CDK Substrates (PXSP or SPXR/K) (34B2) Rabbit mAb Cat#: 2325, PTMScan(R) Phospho-MAPK/CDK Substrate Motif (PXSP, SPXK/R) Immunoaffinity Beads Cat#: 1982 Curated Info
49	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
50	Horiike Y, Kobayashi H, Sekiguchi T (2009) Ran GTPase guanine nucleotide exchange factor RCC1 is phosphorylated on serine 11 by cdc2 kinase in vitro. Mol Biol Rep 36, 717-23 18568422 Curated Info
51	Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7 18669648 Curated Info
52	Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50 18452278 Curated Info
53	Imami K, et al. (2008) Automated Phosphoproteome Analysis for Cultured Cancer Cells by Two-Dimensional NanoLC-MS Using a Calcined Titania/C18 Biphasic Column. Anal Sci 24, 161-6 18187866 Curated Info
54	Hood FE, Clarke PR (2007) RCC1 isoforms differ in their affinity for chromatin, molecular interactions and regulation by phosphorylation. J Cell Sci 120, 3436-45 17855385 Curated Info
55	Wang Y, et al. (2007) Profiling signaling polarity in chemotactic cells. Proc Natl Acad Sci U S A 104, 8328-33 17494752 Curated Info
56	Molina H, et al. (2007) Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry. Proc Natl Acad Sci U S A 104, 2199-204 17287340 Curated Info
57	Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48 17081983 Curated Info
58	Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5 15302935 Curated Info
59	Hutchins JR, et al. (2004) Phosphorylation regulates the dynamic interaction of RCC1 with chromosomes during mitosis. Curr Biol 14, 1099-104 15203004 Curated Info