Ser255

Site Information
LNstGsLsPKHsLkQ SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 451878

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 20 , 21 , 22 , 23 , 24 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 )
Disease tissue studied:	breast cancer ( 5 , 10 , 11 , 22 ) , breast ductal carcinoma ( 10 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 , 10 ) , cervical cancer ( 39 ) , cervical adenocarcinoma ( 39 ) , leukemia ( 26 , 47 , 48 ) , acute myelogenous leukemia ( 26 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 21 ) , acute megakaryoblastic leukemia (M7) ( 21 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 21 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 21 ) , acute myeloblastic leukemia, without maturation (M1) ( 21 ) , chronic myelogenous leukemia ( 47 , 48 ) , hepatocellular carcinoma, surrounding tissue ( 37 ) , lung cancer ( 8 , 16 , 22 , 29 , 32 ) , non-small cell lung cancer ( 22 ) , non-small cell lung adenocarcinoma ( 8 , 16 , 29 ) , lymphoma ( 12 ) , B cell lymphoma ( 21 ) , Burkitt's lymphoma ( 12 ) , non-Hodgkin's lymphoma ( 21 ) , follicular lymphoma ( 12 ) , mantle cell lymphoma ( 12 ) , ovarian cancer ( 10 ) , pancreatic ductal adenocarcinoma ( 15 ) , multiple myeloma ( 21 )
Relevant cell line - cell type - tissue:	'pancreatic, ductal'-pancreas ( 15 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 35 ) , 293 (epithelial) [AT1 (human), transfection] ( 34 ) , 293 (epithelial) ( 41 ) , 293E (epithelial) ( 28 ) , 786-O (renal) [VHL (human), transfection] ( 6 ) , 786-O (renal) ( 6 ) , A498 (renal) ( 36 ) , A549 (pulmonary) ( 17 ) , AML-193 (monocyte) ( 21 ) , BJAB (B lymphocyte) ( 12 ) , breast ( 3 , 10 ) , BT-20 (breast cell) ( 22 ) , BT-474 (breast cell) ( 5 ) , BT-549 (breast cell) ( 22 ) , Calu 6 (pulmonary) ( 22 ) , Chang liver (cervical) ( 50 ) , CL1-0 (pulmonary) ( 32 ) , CL1-1 (pulmonary) ( 32 ) , CL1-2 (pulmonary) ( 32 ) , CL1-5 (pulmonary) ( 32 ) , CMK (megakaryoblast) ( 21 ) , CTS (myeloid) ( 21 ) , DG75 (B lymphocyte) ( 33 ) , DOHH2 ('B lymphocyte, precursor') ( 21 ) , endothelial-aorta ( 23 ) , FL-18 (B lymphocyte) ( 12 ) , FL-318 (B lymphocyte) ( 12 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 24 ) , Flp-In T-Rex-293 (epithelial) ( 24 ) , H2009 (pulmonary) ( 22 ) , H2077 (pulmonary) ( 22 ) , H2887 (pulmonary) ( 22 ) , H322M (pulmonary) ( 22 ) , HCC1359 (pulmonary) ( 22 ) , HCC1937 (breast cell) ( 22 ) , HCC2279 (pulmonary) ( 22 ) , HCC366 (pulmonary) ( 22 ) , HCC4006 (pulmonary) ( 22 ) , HCC78 (pulmonary) ( 22 ) , HCC827 (pulmonary) ( 22 ) , HCT116 (intestinal) ( 42 ) , HEK293T (epithelial) ( 7 ) , HEL (erythroid) ( 21 ) , HeLa (cervical) ( 2 , 9 , 20 , 31 , 40 , 44 , 46 , 49 , 54 , 55 ) , HeLa S3 (cervical) ( 39 ) , hepatocyte-liver ( 37 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 11 ) , HMLER ('stem, breast cancer') ( 11 ) , HOP62 (pulmonary) ( 22 ) , HUES-9 ('stem, embryonic') ( 30 ) , JEKO-1 (B lymphocyte) ( 12 ) , Jurkat (T lymphocyte) ( 18 , 27 , 45 , 51 , 52 , 53 , 56 , 57 ) , K562 (erythroid) ( 20 , 47 , 48 ) , Kasumi-1 (myeloid) ( 21 ) , KG-1 (myeloid) ( 21 , 26 ) , LCLC-103H (pulmonary) ( 22 ) , leukocyte-blood ( 38 ) , liver ( 14 ) , LOU-NH91 (squamous) ( 22 ) , lung ( 16 ) , MCF-7 (breast cell) ( 5 , 22 ) , MDA-MB-231 (breast cell) ( 22 ) , MDA-MB-468 (breast cell) ( 22 ) , MV4-11 (macrophage) ( 21 ) , NCEB-1 (B lymphocyte) ( 12 ) , NCI-H1395 (pulmonary) ( 22 ) , NCI-H1568 (pulmonary) ( 22 ) , NCI-H157 (pulmonary) ( 22 ) , NCI-H1648 (pulmonary) ( 22 ) , NCI-H1666 (pulmonary) ( 22 ) , NCI-H2030 (pulmonary) ( 22 ) , NCI-H2172 (pulmonary) ( 22 ) , NCI-H322 (pulmonary) ( 22 ) , NCI-H460 (pulmonary) ( 22 ) , NCI-H520 (squamous) ( 22 ) , NCI-H647 (pulmonary) ( 22 ) , OCI-ly1 (B lymphocyte) ( 12 ) , OPM-2 (plasma cell) ( 21 ) , ovary ( 10 ) , P31/FUJ (erythroid) ( 21 ) , PC9 (pulmonary) ( 8 , 22 ) , PC9-IR (pulmonary) ( 8 ) , Raji (B lymphocyte) ( 12 ) , RAMOS (B lymphocyte) ( 12 ) , REC-1 (B lymphocyte) ( 12 ) , RL ('B lymphocyte, precursor') ( 21 ) , RPMI-8266 (plasma cell) ( 21 ) , SH-SY5Y (neural crest) ( 13 ) , SU-DHL-4 (B lymphocyte) ( 12 ) , SU-DHL-6 (B lymphocyte) ( 21 ) , U-1810 (pulmonary) [EFNB3 (human), knockdown] ( 29 ) , U266 (plasma cell) ( 21 ) , UPN-1 (B lymphocyte) ( 12 ) , Vero E6-S ('epithelial, kidney') ( 1 ) , WM115 (melanocyte) ( 43 )

Upstream Regulation
Treatments:	angiotensin_2 ( 35 ) , antibody ( 45 ) , EGF ( 40 ) , metastatic potential ( 32 ) , nocodazole ( 39 ) , SII_angiotensin_2 ( 34 , 35 ) , U0126 ( 40 )

References
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29	Ståhl S, et al. (2011) Phosphoproteomic profiling of NSCLC cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. J Proteome Res 10, 2566-78 21413766 Curated Info
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34	Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304 20686112 Curated Info
35	Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53 20363803 Curated Info
36	Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62 20071362 Curated Info
37	Han G, et al. (2010) Phosphoproteome analysis of human liver tissue by long-gradient nanoflow LC coupled with multiple stage MS analysis. Electrophoresis 31, 1080-9 20166139 Curated Info
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39	Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3 20068231 Curated Info
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41	Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501 19413330 Curated Info
42	Nagano K, et al. (2009) Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment. Proteomics 9, 2861-74 19415658 Curated Info
43	Old WM, et al. (2009) Functional proteomics identifies targets of phosphorylation by B-Raf signaling in melanoma. Mol Cell 34, 115-31 19362540 Curated Info
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50	Sui S, et al. (2008) Phosphoproteome analysis of the human Chang liver cells using SCX and a complementary mass spectrometric strategy. Proteomics 8, 2024-34 18491316 Curated Info
51	Stokes M (2008) CST Curation Set: 3884; Year: 2008; Biosample/Treatment: cell line, Jurkat/pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info
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